Evaluation of multiple oxidation products for monitoring effects of antioxidants in Fenton oxidation of 2 '-deoxyguanosine

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7 Citations (SciVal)

Abstract

The objective of this study was to investigate the influence of the two antioxidants, ascorbic acid and (+)catechin, on the oxidation of 2'-deoxyguanosine (dG), using an iron-mediated Fenton reaction. The oxidation products 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'-cyclo-2'-deoxyguanosine, together with the secondary oxidation products guanidinohydantoin and dehydroguanidinohydantoin, were identified and quantified through the use of an LC-MS/MS system. The results obtained showed that catechin inhibited the oxidation better than ascorbic acid did, indicating that the chelating ability of catechin rather than the radical scavenging mechanism alone is vital for the observed antioxidative efficiency. The correlation between the different oxidation products was found to be quite low, primarily because of the instability of 8-oxodG, making it prone to further oxidation. This led to apparent anti- and pro-oxidative results being obtained, emphasizing the potential problems in evaluating oxidative stress, by use of a single marker.
Original languageEnglish
Pages (from-to)2350-2358
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number6
DOIs
Publication statusPublished - 2006

Subject classification (UKÄ)

  • Agricultural Science, Forestry and Fisheries

Keywords

  • ascorbic acid
  • Fenton reaction
  • oxidation
  • 2 '-deoxyguanosine
  • catechin

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