Evaluation of quantitative thin layer chromatography using staining reagents

Richard Johnsson; Gustav Traff; Martin Sundén; Ulf Ellervik

doi:10.1016/j.chroma.2007.07.029

Evaluation of quantitative thin layer chromatography using staining reagents

Richard Johnsson, Gustav Traff, Martin Sundén, Ulf Ellervik

Centre for Analysis and Synthesis

Research output: Contribution to journal › Article › peer-review

Abstract

Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	298-305
Journal	Journal of Chromatography A
Volume	1164
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2007.07.029
Publication status	Published - 2007

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Organic chemistry (S/LTH) (011001240)

Subject classification (UKÄ)

Organic Chemistry

Free keywords

quantification
TLC
image analysis software
staining reagents

Access to Document

10.1016/j.chroma.2007.07.029

Cite this

@article{771476c71dcb42debac8b2a7cfe708ef,

title = "Evaluation of quantitative thin layer chromatography using staining reagents",

abstract = "Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.",

keywords = "quantification, TLC, image analysis software, staining reagents",

author = "Richard Johnsson and Gustav Traff and Martin Sund{\'e}n and Ulf Ellervik",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Organic chemistry (S/LTH) (011001240)",

year = "2007",

doi = "10.1016/j.chroma.2007.07.029",

language = "English",

volume = "1164",

pages = "298--305",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Evaluation of quantitative thin layer chromatography using staining reagents

AU - Johnsson, Richard

AU - Traff, Gustav

AU - Sundén, Martin

AU - Ellervik, Ulf

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Organic chemistry (S/LTH) (011001240)

PY - 2007

Y1 - 2007

N2 - Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.

AB - Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.

KW - quantification

KW - TLC

KW - image analysis software

KW - staining reagents

U2 - 10.1016/j.chroma.2007.07.029

DO - 10.1016/j.chroma.2007.07.029

M3 - Article

SN - 0021-9673

VL - 1164

SP - 298

EP - 305

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Evaluation of quantitative thin layer chromatography using staining reagents

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

Access to Document

Fingerprint

Cite this