Evaluation of simple enzyme kinetics by response surface modelling

Mats Andersson; Patrick Adlercreutz

doi:10.1023/A:1008994613645

Evaluation of simple enzyme kinetics by response surface modelling

Research output: Contribution to journal › Article › peer-review

Abstract

The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.

Original language	English
Pages (from-to)	903-907
Number of pages	5
Journal	Biotechnology Techniques
Volume	13
Issue number	12
DOIs	https://doi.org/10.1023/A:1008994613645
Publication status	Published - 1999 Dec 1

Subject classification (UKÄ)

Biocatalysis and Enzyme Technology

Free keywords

Alcohol dehydrogenase
Cyclohexanone
Enzyme kinetics
Response surface modelling

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10.1023/A:1008994613645

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title = "Evaluation of simple enzyme kinetics by response surface modelling",

abstract = "The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.",

keywords = "Alcohol dehydrogenase, Cyclohexanone, Enzyme kinetics, Response surface modelling",

author = "Mats Andersson and Patrick Adlercreutz",

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AU - Andersson, Mats

AU - Adlercreutz, Patrick

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N2 - The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.

AB - The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.

KW - Alcohol dehydrogenase

KW - Cyclohexanone

KW - Enzyme kinetics

KW - Response surface modelling

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U2 - 10.1023/A:1008994613645

DO - 10.1023/A:1008994613645

M3 - Article

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VL - 13

SP - 903

EP - 907

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 12

ER -

Evaluation of simple enzyme kinetics by response surface modelling

Abstract

Subject classification (UKÄ)

Free keywords

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