Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus

Roya R R Sardari, Evelina Kulcinskaja, Emanuel Y C Ron, Snædís Björnsdóttir, Ólafur H. Friðjónsson, Guðmundur Óli Hreggviðsson, Eva Nordberg Karlsson

Research output: Contribution to journalArticlepeer-review

Abstract

The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252(T) and MAT493 are reported and compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed that the EPS were produced both in the exponential and stationary growth phase and that production in the exponential phase was boosted by maltose supplementation, while stationary phase production was boosted by lactose. The latter was higher, resulting in 8.8 (DSM4252(T)) and 13.7mg EPS/g cell dry weight (MAT493) in cultures in marine broth supplemented with 10g/L lactose. The EPSs were heteropolymeric with an average molecular weight of 8×10(4)Da and different monosaccharides, including arabinose and xylose. FT-IR spectroscopy revealed presence of hydroxyl, carboxyl, N-acetyl, amine, and sulfate ester groups, showing that R. marinus produces unusual sulfated EPS with high arabinose and xylose content.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalCarbohydrate Polymers
Volume156
Early online date2017 Jan 20
DOIs
Publication statusPublished - 2017 Jan 20

Subject classification (UKÄ)

  • Polymer Chemistry

Free keywords

  • EPS
  • Exopolysaccharide
  • Heteropolymer
  • Rhodothermus marinus

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