Abstract

Ex vivo working porcine heart models allow for the study of a heart’s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model’s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model’s ability to rapidly adjust pressure in the large arteries, thereby providing a more accurate and dynamic experimental model. Finally, we provide a comprehensive experimental protocol for the ex vivo working porcine heart model.
Original languageEnglish
Title of host publicationExperimental Models of Cardiovascular Diseases
Subtitle of host publicationMethods and Protocols
EditorsKiyotake Ishikawa
Place of PublicationNew York, NY
PublisherHumana Press
Chapter7
Pages87-107
Number of pages20
Edition2
ISBN (Electronic)978-1-0716-3846-0
ISBN (Print)978-1-0716-3848-4, 978-1-0716-3845-3
DOIs
Publication statusPublished - 2024 Apr 28

Publication series

NameMethods in Molecular Biology
PublisherSpringer Protocols
Volume2803
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Subject classification (UKÄ)

  • Medical Equipment Engineering
  • Medical Biotechnology
  • Cardiac and Cardiovascular Systems
  • Control Engineering

Free keywords

  • Working porcine heart
  • Isolated heart preparation
  • Functional heart evaluation
  • cardiac afterload
  • cyber-physiological systems
  • functional heart assessment
  • ex vivo heart perfusion
  • ex situ heart perfusion
  • ex vivo heart evaluation
  • heart transplantation

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