Expression patterns of the human sarcoma-associated genes FUS and EWS and the genomic structure of FUS

Pierre Åman; Ioannis Panagopoulos; Carin Lassen; Thoas Fioretos; Marina Mencinger; Håkan Toresson; Mattias Höglund; Allan Forster; Terence H. Rabbitts; David Ron; Nils Mandahl; Felix Mitelman

doi:10.1006/geno.1996.0513

Expression patterns of the human sarcoma-associated genes FUS and EWS and the genomic structure of FUS

Pierre Åman, Ioannis Panagopoulos, Carin Lassen, Thoas Fioretos, Marina Mencinger, Håkan Toresson, Mattias Höglund, Allan Forster, Terence H. Rabbitts, David Ron, Nils Mandahl, Felix Mitelman

Research output: Contribution to journal › Article › peer-review

Abstract

FUS (TLS) was first identified as the 5'-part of a fusion gene with CHOP (GADD153, DDIT3) in myxoid liposarcomas with t(12; 16)(q13; p11). Homologies were found with the EWS oncogene, which is rearranged in Ewing sarcomas and other neoplasias. The genomic structure of FUS shows extensive similarities with that of EWS, but the exon/intron structures differ in the 5' parts, and overall FUS is smaller than EWS. Exon 3 of FUS corresponds to exons 3 and 4 in EWS. FUS exons 4-6 correspond to EWS exons 5-8. Exons 7 to 15 of FUS are very similar to those in EWS, although the EWS exons are larger than the corresponding FUS exons. FUS and EWS were expressed in all tissues investigated. The transcripts were stable within the 160-min half-life experiments. No or little variation in FUS or EWS expression was seen when resting lymphocytes were activated. These observations indicate that FUS and EWS belong to the housekeeping type of genes. This view is supported by the presence of the housekeeping gene type of promoter region in both genes.

Original language	English
Pages (from-to)	1-8
Journal	Genomics
Volume	37
Issue number	1
DOIs	https://doi.org/10.1006/geno.1996.0513
Publication status	Published - 1996

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Laboratory for Experimental Brain Research (013041000), Division of Clinical Genetics (013022003)

Subject classification (UKÄ)

Neurology
Medical Genetics

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10.1006/geno.1996.0513

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@article{52cd9ee96bb74614b3558b660d9de7da,

title = "Expression patterns of the human sarcoma-associated genes FUS and EWS and the genomic structure of FUS",

abstract = "FUS (TLS) was first identified as the 5'-part of a fusion gene with CHOP (GADD153, DDIT3) in myxoid liposarcomas with t(12; 16)(q13; p11). Homologies were found with the EWS oncogene, which is rearranged in Ewing sarcomas and other neoplasias. The genomic structure of FUS shows extensive similarities with that of EWS, but the exon/intron structures differ in the 5' parts, and overall FUS is smaller than EWS. Exon 3 of FUS corresponds to exons 3 and 4 in EWS. FUS exons 4-6 correspond to EWS exons 5-8. Exons 7 to 15 of FUS are very similar to those in EWS, although the EWS exons are larger than the corresponding FUS exons. FUS and EWS were expressed in all tissues investigated. The transcripts were stable within the 160-min half-life experiments. No or little variation in FUS or EWS expression was seen when resting lymphocytes were activated. These observations indicate that FUS and EWS belong to the housekeeping type of genes. This view is supported by the presence of the housekeeping gene type of promoter region in both genes.",

author = "Pierre {\AA}man and Ioannis Panagopoulos and Carin Lassen and Thoas Fioretos and Marina Mencinger and H{\aa}kan Toresson and Mattias H{\"o}glund and Allan Forster and Rabbitts, {Terence H.} and David Ron and Nils Mandahl and Felix Mitelman",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Laboratory for Experimental Brain Research (013041000), Division of Clinical Genetics (013022003)",

year = "1996",

doi = "10.1006/geno.1996.0513",

language = "English",

volume = "37",

pages = "1--8",

journal = "Genomics",

issn = "1089-8646",

publisher = "Academic Press",

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TY - JOUR

T1 - Expression patterns of the human sarcoma-associated genes FUS and EWS and the genomic structure of FUS

AU - Åman, Pierre

AU - Panagopoulos, Ioannis

AU - Lassen, Carin

AU - Fioretos, Thoas

AU - Mencinger, Marina

AU - Toresson, Håkan

AU - Höglund, Mattias

AU - Forster, Allan

AU - Rabbitts, Terence H.

AU - Ron, David

AU - Mandahl, Nils

AU - Mitelman, Felix

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Laboratory for Experimental Brain Research (013041000), Division of Clinical Genetics (013022003)

PY - 1996

Y1 - 1996

N2 - FUS (TLS) was first identified as the 5'-part of a fusion gene with CHOP (GADD153, DDIT3) in myxoid liposarcomas with t(12; 16)(q13; p11). Homologies were found with the EWS oncogene, which is rearranged in Ewing sarcomas and other neoplasias. The genomic structure of FUS shows extensive similarities with that of EWS, but the exon/intron structures differ in the 5' parts, and overall FUS is smaller than EWS. Exon 3 of FUS corresponds to exons 3 and 4 in EWS. FUS exons 4-6 correspond to EWS exons 5-8. Exons 7 to 15 of FUS are very similar to those in EWS, although the EWS exons are larger than the corresponding FUS exons. FUS and EWS were expressed in all tissues investigated. The transcripts were stable within the 160-min half-life experiments. No or little variation in FUS or EWS expression was seen when resting lymphocytes were activated. These observations indicate that FUS and EWS belong to the housekeeping type of genes. This view is supported by the presence of the housekeeping gene type of promoter region in both genes.

AB - FUS (TLS) was first identified as the 5'-part of a fusion gene with CHOP (GADD153, DDIT3) in myxoid liposarcomas with t(12; 16)(q13; p11). Homologies were found with the EWS oncogene, which is rearranged in Ewing sarcomas and other neoplasias. The genomic structure of FUS shows extensive similarities with that of EWS, but the exon/intron structures differ in the 5' parts, and overall FUS is smaller than EWS. Exon 3 of FUS corresponds to exons 3 and 4 in EWS. FUS exons 4-6 correspond to EWS exons 5-8. Exons 7 to 15 of FUS are very similar to those in EWS, although the EWS exons are larger than the corresponding FUS exons. FUS and EWS were expressed in all tissues investigated. The transcripts were stable within the 160-min half-life experiments. No or little variation in FUS or EWS expression was seen when resting lymphocytes were activated. These observations indicate that FUS and EWS belong to the housekeeping type of genes. This view is supported by the presence of the housekeeping gene type of promoter region in both genes.

U2 - 10.1006/geno.1996.0513

DO - 10.1006/geno.1996.0513

M3 - Article

SN - 1089-8646

VL - 37

SP - 1

EP - 8

JO - Genomics

JF - Genomics

IS - 1

ER -

Expression patterns of the human sarcoma-associated genes FUS and EWS and the genomic structure of FUS

Abstract

Bibliographical note

Subject classification (UKÄ)

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