Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in Bacillus subtilis

Research output: Contribution to journalArticlepeer-review

Abstract

The trxA gene is regarded as essential in Bacillus subtilis, but the roles of the TrxA protein in this gram-positive bacterium are largely unknown. Inactivation of trxA results in deoxyribonucleoside and cysteine or methionine auxotrophy. This phenotype is expected if the TrxA protein is important for the activity of the class Ib ribonucleotide reductase and adenosine-5'-phosphosulfate/3'-phosphoadenosine-5'-phosphosulfate reductase. We demonstrate here that a TrxA deficiency in addition causes defects in endospore and cytochrome c synthesis. These effects were suppressed by BdbD deficiency, indicating that TrxA in the cytoplasm is the primary electron donor to several different thiol-disulfide oxidoreductases active on the outer side of the B. subtilis cytoplasmic membrane.
Original languageEnglish
Pages (from-to)4660-4665
JournalJournal of Bacteriology
Volume190
Issue number13
DOIs
Publication statusPublished - 2008

Subject classification (UKÄ)

  • Microbiology
  • Biochemistry and Molecular Biology

Fingerprint

Dive into the research topics of 'Extracytoplasmatic processes impaired by inactivation of the trxA (thioredoxin gene) in Bacillus subtilis'. Together they form a unique fingerprint.

Cite this