Facile Pretreatment of Bacilius circulans beta-Galactosidase Increases the Yield of Galactosyl Oligosaccharides in Milk and Lactose Reaction Systems

Aaron Gosling, Johan Alftrén, Geoff W. Stevens, Andrew R. Barber, Sandra E. Kentish, Sally L. Gras

Research output: Contribution to journalArticlepeer-review

37 Citations (SciVal)

Abstract

The commercially available preparation of beta-galactosidase from Bacillus circulans, known as Biolacta FN5, has been extensively used in the production of prebiotic galactooligosaccharides (GOS). This study focuses on characterizing the production of GOS in two reaction systems: 10% lactose (w/v) in buffer and skim milk. Analysis of the temperature dependence of the GOS yield along with the relative rates of GOS synthesis and degradation leads to the finding that GOS degradation activity was selectively decreased in Biolacta FN5 above 40 degrees C. Facile heat treatment of Biolacta FN5 solution prior to use allowed for GOS yields to be significantly increased in both reaction systems.
Original languageEnglish
Pages (from-to)11570-11574
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number24
DOIs
Publication statusPublished - 2009

Subject classification (UKÄ)

  • Agricultural Science, Forestry and Fisheries

Keywords

  • beta-galactosidase
  • galactosyl transfer
  • prebiotic
  • Galactooligosaccharides
  • GOS
  • lactose hydrolysis
  • Bacillus circulans

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