Fast determination of whole blood glucose with a calorimetric micro-biosensor

Bin Xie; Ulrika Hedberg; Michael Mecklenburg; Bengt Danielsson

doi:10.1016/0925-4005(93)85040-H

Fast determination of whole blood glucose with a calorimetric micro-biosensor

Bin Xie, Ulrika Hedberg, Michael Mecklenburg, Bengt Danielsson

Pure and Applied Biochemistry

Lund University

Research output: Contribution to journal › Article › peer-review

Abstract

Fast determination of whole blood glucose without any pretreatment was achieved using a calorimetric FIA micro-biosensor. Glucose oxidase was covalently immobilized, together with catalase, onto controlled pore glass beads and packed into a micro-column. By reducing the sample volume down to 1 μ1, a measurement period of about 40 s and an enlarged linear range of 0.5-20 mM glucose were obtained. The correlation coefficient was 0.98 when the micro-biosensor was compared to the Reflolux-S blood glucose analyser. The relative standard deviation for 100 blood samples was 3.7%.

Original language	English
Pages (from-to)	141-144
Number of pages	4
Journal	Sensors and Actuators B: Chemical
Volume	15
Issue number	1-3
DOIs	https://doi.org/10.1016/0925-4005(93)85040-H
Publication status	Published - 1993 Aug

Subject classification (UKÄ)

Analytical Chemistry

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10.1016/0925-4005(93)85040-H

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title = "Fast determination of whole blood glucose with a calorimetric micro-biosensor",

abstract = "Fast determination of whole blood glucose without any pretreatment was achieved using a calorimetric FIA micro-biosensor. Glucose oxidase was covalently immobilized, together with catalase, onto controlled pore glass beads and packed into a micro-column. By reducing the sample volume down to 1 μ1, a measurement period of about 40 s and an enlarged linear range of 0.5-20 mM glucose were obtained. The correlation coefficient was 0.98 when the micro-biosensor was compared to the Reflolux-S blood glucose analyser. The relative standard deviation for 100 blood samples was 3.7%.",

author = "Bin Xie and Ulrika Hedberg and Michael Mecklenburg and Bengt Danielsson",

year = "1993",

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doi = "10.1016/0925-4005(93)85040-H",

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AU - Xie, Bin

AU - Hedberg, Ulrika

AU - Mecklenburg, Michael

AU - Danielsson, Bengt

PY - 1993/8

Y1 - 1993/8

N2 - Fast determination of whole blood glucose without any pretreatment was achieved using a calorimetric FIA micro-biosensor. Glucose oxidase was covalently immobilized, together with catalase, onto controlled pore glass beads and packed into a micro-column. By reducing the sample volume down to 1 μ1, a measurement period of about 40 s and an enlarged linear range of 0.5-20 mM glucose were obtained. The correlation coefficient was 0.98 when the micro-biosensor was compared to the Reflolux-S blood glucose analyser. The relative standard deviation for 100 blood samples was 3.7%.

AB - Fast determination of whole blood glucose without any pretreatment was achieved using a calorimetric FIA micro-biosensor. Glucose oxidase was covalently immobilized, together with catalase, onto controlled pore glass beads and packed into a micro-column. By reducing the sample volume down to 1 μ1, a measurement period of about 40 s and an enlarged linear range of 0.5-20 mM glucose were obtained. The correlation coefficient was 0.98 when the micro-biosensor was compared to the Reflolux-S blood glucose analyser. The relative standard deviation for 100 blood samples was 3.7%.

U2 - 10.1016/0925-4005(93)85040-H

DO - 10.1016/0925-4005(93)85040-H

M3 - Article

AN - SCOPUS:0027647443

SN - 0925-4005

VL - 15

SP - 141

EP - 144

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 1-3

ER -

Fast determination of whole blood glucose with a calorimetric micro-biosensor

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