Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species

Mattias Gustafsson, Colin N. Palmer, Roland C. Wolf, Claes von Wachenfeldt

Research output: Contribution to journalArticlepeer-review

21 Citations (SciVal)

Abstract

Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR encodes a protein that binds to an operator sequence that is present upstream of its own reading frame, thereby repressing the expression of the fatR-cyp102A3 operon. Unsaturated fatty acids and phytanic acid have the capacity to interact with FatR and to abrogate its binding to the operator sequence.
Original languageEnglish
Pages (from-to)459-464
JournalArchives of Microbiology
Volume176
Issue number6
DOIs
Publication statusPublished - 2001

Subject classification (UKÄ)

  • Immunology in the medical area
  • Microbiology in the medical area

Keywords

  • Cyp102A2
  • Cyp102A1
  • FatR
  • Bacillus subtilis
  • cytochrome P450
  • P450BM-3
  • Cyp102A3

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