Abstract
We could determine the S-phase fraction (SPF) by flow cytometric DNA analysis of paraffin archival material in 160 of 260 patients with soft-tissue sarcoma of extremity and trunk wall. The prognostic value of SPF was compared with other clinicopathological factors. The median follow-up time was 16 (6-31) years. In a univariate analysis, deep tumour location, increasing tumour size and histological malignancy grade, microscopic tumour necrosis, vascular invasion, DNA non-diploidy and high SPF (>3.0%) were associated with poor metastasis-free survival. In a multivariate analysis, microscopic tumour necrosis and high SPF were independently prognostic for metastasis. Used in combination with tumour size, microscopic tumour necrosis and vascular invasion, SPF could identify a group of patients with a 5-year metastasis-free survival rate of 0.97. This group constituted one-quarter of all patients. Patients with low SPF who did recur had a prolonged clinical course both as regards metastases and local recurrence. We conclude that SPF is a valuable adjunct in prognostication in soft-tissue sarcoma.
| Original language | English |
|---|---|
| Pages (from-to) | 94-100 |
| Journal | British Journal of Cancer |
| Volume | 75 |
| Issue number | 1 |
| Publication status | Published - 1997 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Pathology, (Lund) (013030000), Department of Orthopaedics (Lund) (013028000), Oncology, MV (013035000)
Subject classification (UKÄ)
- Cancer and Oncology
Free keywords
- Soft tissue
- Sarcoma
- S Phase
- Flow cytometry
- Cell cycle
- DNA
- Ploidy
- Prognosis
- Human
- Malignant tumor
