Abstract
Detection of P. falciparum-specific subpopulations of B-cells is important for studies of immunity in malaria. This protocol relies on the photostability and protein loading capacity of carboxylated quantum dots to detect a broad range of different P. falciparum-specific B-cells. Infected red blood cell ghosts, obtained by permeabilization of infected cells with Streptolysin O, are coupled with carboxyl quantum dots using N-ethyl-N-dimethylaminopropyl-carbodiimide condensation. Immunophenotyping of P. falciparum-specific B-cells is performed by flow cytometry using Fc-receptor block, quantum dot-infected red blood cell ghost conjugates, and fluorochrome-conjugated anti-human CD19 mouse monoclonal antibodies.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press |
| Pages | 681-688 |
| Number of pages | 8 |
| ISBN (Electronic) | 978-1-0716-2189-9 |
| DOIs | |
| Publication status | Published - 2022 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2470 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Subject classification (UKÄ)
- Immunology
- Immunology in the Medical Area (including Cell and Immunotherapy)
Free keywords
- B-cells
- Flow cytometry
- Ghost infected red blood cells
- Malaria
- Plasmodium falciparum
- Quantum dots
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