Flow FFF–basics and key applications

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The 1990s and 2000s have seen a rapidly growing use of flow field-flow fractionation (flow FFF, FlFFF). As of today hundreds of publications in many different application areas are presented each year in which flow FFF has been used or is referred to. In this chapter a brief historical overview of flow FFF is given. Channel designs and basic principles are discussed as well as approaches to development of rapid high resolution separations. Finally, an overview of key applications is included with pioneering and ground-breaking papers from literature.
Original languageEnglish
Title of host publicationField-flow fractionation in biopolymer analysis
EditorsS. Kim R. Williams, Karin D. Caldwell
Number of pages21
ISBN (Electronic)978-3-7091-0154-4
ISBN (Print)978-3-7091-0153-7
Publication statusPublished - 2012

Subject classification (UKÄ)

  • Analytical Chemistry

Free keywords

  • Flow field-flow fractionation
  • Flow FFF
  • Asymmetrical flow FFF
  • Protein aggregates
  • Plasmids
  • High resolution
  • Rapid separations
  • H-value
  • Time-average velocity
  • Velocity gradient
  • Polysaccharides
  • Ultra-high molar mass
  • Zone broadening


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