Fluorescence Microscopy of Nanochannel-Confined DNA

Sriram Kk, Fredrik Persson, Joachim Fritzsche, Jason P. Beech, Jonas O. Tegenfeldt, Fredrik Westerlund

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Place of PublicationNew York
PublisherHumana Press
Pages175-202
Number of pages28
Volume2694
ISBN (Electronic)978-1-0716-3377-9
ISBN (Print)978-1-0716-3376-2
DOIs
Publication statusPublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2694
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Subject classification (UKÄ)

  • Other Physics Topics

Free keywords

  • DNA
  • Fluorescence
  • Nanochannels
  • Single molecule

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