TY - JOUR
T1 - Fluorescence polarisation for immunoreagent characterisation
AU - Önnerfjord, P.
AU - Eremin, S.
AU - Emnéus, J.
AU - Marko-Varga, G.
PY - 1998/4/1
Y1 - 1998/4/1
N2 - Antibodies were characterised using fluorescence polarisation, a homogeneous assay technique in which all reagents are in solution. Kinetic studies on the association and dissociation of the immunocomplex were performed. A competitive assay was used and the sensitivities, operational linearities, as well as the specificities of the immunoassays were experimentally determined for various antibody preparations with specificity for triazines. Detection limits for atrazine in water samples were determined to be within the range of 0.08-0.4 ng ml-1 using a 5-min incubation time and a 0.5-ml sample volume.
AB - Antibodies were characterised using fluorescence polarisation, a homogeneous assay technique in which all reagents are in solution. Kinetic studies on the association and dissociation of the immunocomplex were performed. A competitive assay was used and the sensitivities, operational linearities, as well as the specificities of the immunoassays were experimentally determined for various antibody preparations with specificity for triazines. Detection limits for atrazine in water samples were determined to be within the range of 0.08-0.4 ng ml-1 using a 5-min incubation time and a 0.5-ml sample volume.
KW - Antibody characterisation
KW - Atrazine
KW - Fluorescence polarisation
KW - Homogenous immunoassay
KW - Triazines
UR - http://www.scopus.com/inward/record.url?scp=0031845984&partnerID=8YFLogxK
U2 - 10.1016/S0022-1759(98)00019-2
DO - 10.1016/S0022-1759(98)00019-2
M3 - Article
C2 - 9671123
AN - SCOPUS:0031845984
SN - 0022-1759
VL - 213
SP - 31
EP - 39
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -