Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging

Jenny Svensson, A Garofalakis, H Meyer, Florian Forster, J Ripoll, Stefan Andersson-Engels

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceedingpeer-review

Abstract

A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a ratio of yellow to red fluorescence was calculated for several depths in tissue with a thickness of 2 cm. The ratio showed a clear dependence on the depth of the object
Original languageEnglish
Title of host publicationProceedings of the SPIE - The International Society for Optical Engineering
PublisherSPIE
Pages585906-1-585906-6
Volume5859
DOIs
Publication statusPublished - 2005
EventPhoton Migration and Diffuse-Light Imaging II - Munich, Germany
Duration: 2005 Jun 12 → …

Publication series

Name
Number1
Volume5859
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferencePhoton Migration and Diffuse-Light Imaging II
Country/TerritoryGermany
CityMunich
Period2005/06/12 → …

Subject classification (UKÄ)

  • Atom and Molecular Physics and Optics

Free keywords

  • 2 cm
  • yellow fluorescence
  • fluorophores
  • red fluorescence
  • phantom measurements
  • optical tomography
  • fluorescent light detection
  • fluorescent probe
  • deep-lying tumors
  • fluorescent object
  • tissue imaging
  • depth resolution
  • fluorescence spectroscopy
  • tissue phantoms

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