Projects per year
Focal adhesions are force sensitive structures that dynamically alter their composition, protein-protein interactions, and signaling in response to external mechanical stimuli. These dynamic changes are critical for focal adhesion function and are required for cellular mechanosensing. Here, we describe a simple protocol that allows for isolation of the focal adhesion complex from adherent cells in culture in response to different mechanical stimuli applied at adhesion sites. By combining this assay with approaches such as proteomics or western blot analysis, one can study the force-dependent changes in focal adhesion composition, protein-protein interactions and signaling.
|Number of pages||6|
|Journal||Methods in molecular biology (Clifton, N.J.)|
|Publication status||Published - 2021|
Subject classification (UKÄ)
- Cell and Molecular Biology
- Cell Biology
- Focal adhesion composition
FingerprintDive into the research topics of 'Focal Adhesion Isolation Assay Using ECM-Coated Magnetic Beads'. Together they form a unique fingerprint.
- 1 Active
Unravelling the mechanoadhesome of migratory cells
Sturgess, W., Swaminathan, V. & Malmström, J.
2020/02/01 → …