Abstract
A simple and inexpensive amperometric biosensor for formate detection was obtained by immobilization of for-mate dehydrogenase (FDH) on graphite powder, modified with a new phenothiazine derivative (DDDP; 16H, 18H-dibenzo[c,l]-7,9-dithia-16,18-diazapentacene). Two different enzyme environments were used: FDH was adsorbed on DDDP-modified graphite powder in the presence of polyethylenimine (PEI) as well as in its absence. In both cases, when 10 mM NAD(+) were added in the supporting electrolyte (phosphate buffer, pH 7.0), the biosensor developed a response to formate, obeying Michaelis-Menten kinetics with a linear domain up to 3 mM formate. The biosensor sensitivity, calculated as the ratio 1(max)/K-M(aPP), was better in the second case (0.962 mA/M), but the best detection limit (3 AM) was observed in the first case.
| Original language | English |
|---|---|
| Pages (from-to) | 25-30 |
| Journal | Revue Roumaine de Chimie |
| Volume | 51 |
| Issue number | 1 |
| Publication status | Published - 2006 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
Subject classification (UKÄ)
- Analytical Chemistry