Abstract
An aqueous two-phase system based on the two polymers poly(ethylene glycol) and dextran has been used for the fractionation of cellulase enzymes present in culture liquid obtained by fermentation with Trichoderma reesei. The activities of beta-glucosidase and glucanases were separated to high degree by using the two-phase systems for a counter-current distribution process in nine transfer steps. While the glucanases had high affinity to the poly(ethylene glycol) rich top phase the beta-glucosidase was enriched in the dextran-containing bottom phase. Multiple counter-current distribution performed indicates the heterogeneity of beta-glucosidase activities assuming at least four isoenzyme forms. One step concentration of beta-glucosidase by using system with 46:1 phase volume ratio resulted in 16 times higher enzyme activity.
| Original language | English |
|---|---|
| Pages (from-to) | 287-295 |
| Journal | Bioseparation |
| Volume | 7 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1999 |
Subject classification (UKÄ)
- Biological Sciences
Free keywords
- aqueous two-phase systems
- beta-glucosidase
- cellulases
- fractionation of enzymes
- partitioning of proteins
- Trichoderma reesei