Free cytosolic calcium during spontaneous contractions in smooth muscle of the guinea-pig mesotubarium

B Himpens, M L Lydrup, Per Hellstrand, R Casteels

Research output: Contribution to journalArticlepeer-review

Abstract

The free intracellular calcium ion concentration ([Ca2+]i) was measured simultaneously with isometric force in strips of guinea-pig mesotubarium using the Fura-2 technique. During the relaxed period (5-15 min) between spontaneous contractions [Ca2+]i continues to decrease after full mechanical relaxation to reach a minimal level of 86 +/- 8 nM (n = 9) just before the start of the next contraction. During the spontaneous contractions (5-15 min) [Ca2+]i reached a maximum of 211 +/- 19 nM and then oscillated between 155 +/- 16 nM and 194 +/- 9 nM. Increased extracellular Ca2+ concentration to 10 mM from the standard concentration of 1.5 mM caused a decreased frequency of spontaneous contractions and an increase in [Ca2+]i both in the relaxed and contracted states. In 10 mM extracellular Ca2+, addition of AlF4-, as 1 mM NaF + 10 microM AlCl3, caused a sustained increase in [Ca2+]i and maintained force. Addition of verapamil (10 microM) in this situation decreased [Ca2+]i to the resting level. The results suggest that the cyclic appearance of trains of action potentials is related to variation in [Ca2+]i, possibly via inactivation of Ca2(+)-dependent K+ channels.
Original languageEnglish
Pages (from-to)404-409
JournalPflügers Archiv
Volume417
Issue number4
DOIs
Publication statusPublished - 1990

Subject classification (UKÄ)

  • Physiology

Free keywords

  • [Ca2+]i
  • Smooth muscle
  • Mesotubarium
  • Pacemaker activity

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