From gene expression analysis to tissue microarrays - A rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies

Sara Ek, Ulrika Andreasson, S Hober, C Kampf, F Ponten, M Uhlen, H Merz, Carl Borrebaeck

Research output: Contribution to journalArticlepeer-review

Abstract

Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities.
Original languageEnglish
Pages (from-to)1072-1081
JournalMolecular & Cellular Proteomics
Volume5
Issue number6
DOIs
Publication statusPublished - 2006

Subject classification (UKÄ)

  • Immunology in the medical area

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