Abstract
The aim of this study was to examine dose-dependent effects of intranasal application of neuropeptide Y (NPY) on nasal mucosal blood flow, blood content, and intranasal nitric oxide (NO) concentration. Blood flow was measured by laser Doppler flowmetry (LDF) and blood content by rhinomanometry. Mucosal biopsies were taken for investigation of Y1 and Y2 receptor mRNA expression, using the reverse transcriptase-polymerase chain reaction (RT-PCR). Intranasal application of NPY evoked a dose-dependent reduction of nasal mucosal blood flow. Maximal vasoconstriction, seen at 12 nmol, was -37.5 +/- 6.2%, p < 0.05 (n = 9). The vasoconstrictive effect developed within 2 to 4 min and lasted > 17 min. NPY evoked a dose-dependent reduction of nasal airway resistance (NAR) on the ipsilateral side. Maximal decrease was -24.0 +/- 10.0% at 12 nmol, p < 0.05 (n = 9). There was a decrease in nasal NO production on the ipsilateral side after application of NPY 12 nmol (-7.4 +/- 1.2%, p < 0.05, n = 8). RT-PCR products corresponding to Y1 receptor but not Y2 receptor mRNA were obtained from biopsies of the nasal mucosa. In conclusion, NPY is a potent vasoconstrictor in the human nose reducing mucosal blood flow, as well as the blood content. The effect is probably mediated via Y1 receptors. NPY receptor agonists may prove beneficial in the treatment of the congested nose in allergic or vasomotor rhinitis.
Original language | English |
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Pages (from-to) | 1724-1728 |
Number of pages | 5 |
Journal | American Journal of Respiratory and Critical Care Medicine |
Volume | 160 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1999 |
Subject classification (UKÄ)
- Respiratory Medicine and Allergy
Free keywords
- Administration, Intranasal
- Adult
- Airway Resistance/drug effects
- Dose-Response Relationship, Drug
- Female
- Humans
- Laser-Doppler Flowmetry
- Male
- Nasal Mucosa/blood supply
- Neuropeptide Y/administration & dosage
- Nitric Oxide/metabolism
- RNA, Messenger/analysis
- Receptors, Neuropeptide Y/metabolism
- Regional Blood Flow/drug effects
- Reverse Transcriptase Polymerase Chain Reaction
- Vasoconstriction/drug effects