Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose

Muhammad H. Asif, Syed M. Usman Ali, Omer Nur, Magnus Willander, Cecilia Brannmark, Peter Stralfors, Ulrika H. Englund, Fredrik Elinder, Bengt Danielsson

Research output: Contribution to journalArticlepeer-review

110 Citations (SciVal)

Abstract

In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 mu m diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 mu M). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration. (C) 2010 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)2205-2211
JournalBiosensors & Bioelectronics
Volume25
Issue number10
DOIs
Publication statusPublished - 2010

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology

Keywords

  • Intracellular glucose
  • ZnO nanorods
  • Functionalisation
  • sensor
  • Electrochemical

Fingerprint

Dive into the research topics of 'Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose'. Together they form a unique fingerprint.

Cite this