Abstract
The application of a quinoprotein glucose dehydrogenase modified thick-film sensor as label detector in a capillary immunoassay (CIA) for xenoestrogens is presented. The detection of the alkylphenols and their ethoxylates is based on the competition between the analyte and tracer molecules for the binding sites of anti-alkylphenol ethoxylate antibodies. This assay is performed off-line in small disposable PVC capillaries coated with immobilized antibodies. This format allows the combination of the assay with a small portable device potentially useful for on-site environmental monitoring. Beside high amplification the utilization of beta-galactosidase as enzyme label allows the direct combination with a GDH biosensor at optimal pH conditions. The bioelectrocatalytic properties of this biosensor offer an additional amplification and thus allow a very sensitive quantification of 4-aminophenol, generated by the beta-galactosidase. Detection limits of the analytes in the mug/l range were obtained, while other phenolics and surfactants showed no or very little cross reactivity.
| Original language | English |
|---|---|
| Pages (from-to) | 1033-1043 |
| Journal | Biosensors & Bioelectronics |
| Volume | 17 |
| Issue number | 11-12 |
| DOIs | |
| Publication status | Published - 2002 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
Subject classification (UKÄ)
- Analytical Chemistry
Free keywords
- beta-galactosidase
- portable device
- GDH biosensor
- capillary immunoassay
- electrochemical detection
- alkylphenols
- alkylphenol
- ethoxylates