Gemini surfactants: New synthetic vectors for gene transfection

A J Kirby; P Camilleri; J Engberts; M. C. Feiters; R J M Nolte; Olle Söderman; M Bergsma; P C Bell; M L Fielden; C L G Rodriguez; P Guedat; A Kremer; C McGregor; C Perrin; G Ronsin; Marcel C P van Eijk

doi:10.1002/anie.200201597

Gemini surfactants: New synthetic vectors for gene transfection

A J Kirby, P Camilleri, J Engberts, M. C. Feiters, R J M Nolte, Olle Söderman, M Bergsma, P C Bell, M L Fielden, C L G Rodriguez, P Guedat, A Kremer, C McGregor, C Perrin, G Ronsin, Marcel C P van Eijk

Physical Chemistry

Research output: Contribution to journal › Review article › peer-review

Abstract

The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the lipoplex before entry into the nucleus, where the new gene can be expressed with high efficiency.

Original language	English
Pages (from-to)	1448-1457
Journal	Angewandte Chemie International Edition in English
Volume	42
Issue number	13
DOIs	https://doi.org/10.1002/anie.200201597
Publication status	Published - 2003

Subject classification (UKÄ)

Physical Chemistry

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10.1002/anie.200201597

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Kirby, A. J., Camilleri, P., Engberts, J., Feiters, M. C., Nolte, R. J. M., Söderman, O., Bergsma, M., Bell, P. C., Fielden, M. L., Rodriguez, C. L. G., Guedat, P., Kremer, A., McGregor, C., Perrin, C., Ronsin, G., & van Eijk, M. C. P. (2003). Gemini surfactants: New synthetic vectors for gene transfection. Angewandte Chemie International Edition in English, 42(13), 1448-1457. https://doi.org/10.1002/anie.200201597

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title = "Gemini surfactants: New synthetic vectors for gene transfection",

abstract = "The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the lipoplex before entry into the nucleus, where the new gene can be expressed with high efficiency.",

author = "Kirby, {A J} and P Camilleri and J Engberts and Feiters, {M. C.} and Nolte, {R J M} and Olle S{\"o}derman and M Bergsma and Bell, {P C} and Fielden, {M L} and Rodriguez, {C L G} and P Guedat and A Kremer and C McGregor and C Perrin and G Ronsin and {van Eijk}, {Marcel C P}",

year = "2003",

doi = "10.1002/anie.200201597",

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volume = "42",

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journal = "Angewandte Chemie International Edition in English",

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Kirby, AJ, Camilleri, P, Engberts, J, Feiters, MC, Nolte, RJM, Söderman, O, Bergsma, M, Bell, PC, Fielden, ML, Rodriguez, CLG, Guedat, P, Kremer, A, McGregor, C, Perrin, C, Ronsin, G & van Eijk, MCP 2003, 'Gemini surfactants: New synthetic vectors for gene transfection', Angewandte Chemie International Edition in English, vol. 42, no. 13, pp. 1448-1457. https://doi.org/10.1002/anie.200201597

TY - JOUR

T1 - Gemini surfactants: New synthetic vectors for gene transfection

AU - Kirby, A J

AU - Camilleri, P

AU - Engberts, J

AU - Feiters, M. C.

AU - Nolte, R J M

AU - Söderman, Olle

AU - Bergsma, M

AU - Bell, P C

AU - Fielden, M L

AU - Rodriguez, C L G

AU - Guedat, P

AU - Kremer, A

AU - McGregor, C

AU - Perrin, C

AU - Ronsin, G

AU - van Eijk, Marcel C P

PY - 2003

Y1 - 2003

N2 - The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the lipoplex before entry into the nucleus, where the new gene can be expressed with high efficiency.

AB - The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the lipoplex before entry into the nucleus, where the new gene can be expressed with high efficiency.

U2 - 10.1002/anie.200201597

DO - 10.1002/anie.200201597

M3 - Review article

SN - 0570-0833

VL - 42

SP - 1448

EP - 1457

JO - Angewandte Chemie International Edition in English

JF - Angewandte Chemie International Edition in English

IS - 13

ER -

Gemini surfactants: New synthetic vectors for gene transfection

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