@article{6f7d20f4c6464fb0b086a32f176797cd,
title = "Gene expression profiling demonstrates that TGF-{beta}1 signals exclusively through receptor complexes involving Alk5 and identifies targets of TGF-{beta} signaling.",
abstract = "Transforming growth factor-β 1 (TGF-β) regulates cellular functions like proliferation, differentiation, and apoptosis. On the cell surface, TGF-β binds to receptor complexes consisting of TGF-β receptor type II (Tβ RII) and activin-like kinase receptor-5 (Alk5), and the downstream signaling is transduced by Smad and MAPK proteins. Recent data have shown that alternative receptor combinations aside from the classical pairing of Tβ RII/Alk5 can be relevant for TGF-β signaling. We have screened for alternative receptors for TGF-β and also for gene targets of TGF-β signaling, by performing functional assays and microarray analysis in murine embryonic fibroblast (MEF) cell lines lacking Alk5. Data from TGF-β-stimulated Alk5(-/-) cells show them to be completely unaffected by TGF-β. Additionally, 465 downstream targets of Alk5 signaling were identified when comparing Alk5(-/-) or TGF-β-stimulated Alk5(+/+) MEFs with unstimulated Alk5(+/+) cells. Our results demonstrate that, in MEFs, TGF-β signals exclusively through complexes involving Alk5, and give insight to its downstream effector genes.",
keywords = "signal transduction, Smad, microarrays",
author = "G{\"o}ran Karlsson and Yingchun Liu and Jonas Larsson and Marie-Jose Goumans and Ju-Seog Lee and Thorgeirsson, {Snorri S} and Markus Ringn{\'e}r and Stefan Karlsson",
year = "2005",
month = may,
day = "11",
doi = "10.1152/physiolgenomics.00303.2004",
language = "English",
volume = "21",
pages = "396--403",
journal = "Physiological Genomics",
issn = "1094-8341",
publisher = "American Physiological Society",
number = "3",
}