Generation and analyses of human synthetic antibody libraries and their application for protein microarrays

Anna Säll, Maria Walle, Christer Wingren, Susanne Müller, Tomas Nyman, Andrea Vala, Mats Ohlin, Carl A K Borrebaeck, Helena Persson

Research output: Contribution to journalArticlepeer-review


Antibody-based proteomics offers distinct advantages in the analysis of complex samples for discovery and validation of biomarkers associated with disease. However, its large-scale implementation requires tools and technologies that allow development of suitable antibody or antibody fragments in a high-throughput manner. To address this we designed and constructed two human synthetic antibody fragment (scFv) libraries denoted HelL-11 and HelL-13. By the use of phage display technology, in total 466 unique scFv antibodies specific for 114 different antigens were generated. The specificities of these antibodies were analyzed in a variety of immunochemical assays and a subset was further evaluated for functionality in protein microarray applications. This high-throughput approach demonstrates the ability to rapidly generate a wealth of reagents not only for proteome research, but potentially also for diagnostics and therapeutics. In addition, this work provides a great example on how a synthetic approach can be used to optimize library designs. By having precise control of the diversity introduced into the antigen-binding sites, synthetic libraries offer increased understanding of how different diversity contributes to antibody binding reactivity and stability, thereby providing the key to future library optimization.

Original languageEnglish
Pages (from-to)427-437
Number of pages11
JournalProtein Engineering Design & Selection
Issue number10
Publication statusPublished - 2016 Oct 1

Subject classification (UKÄ)

  • Medical Biotechnology

Free keywords

  • affinity proteomics
  • phage display technology
  • protein microarrays
  • scFv
  • synthetic antibody libraries


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