TY - JOUR
T1 - Genetic variation associated with chromosomal aberration frequency
T2 - A genome-wide association study
AU - Niazi, Yasmeen
AU - Thomsen, Hauke
AU - Smolkova, Bozena
AU - Vodickova, Ludmila
AU - Vodenkova, Sona
AU - Kroupa, Michal
AU - Vymetalkova, Veronika
AU - Kazimirova, Alena
AU - Barancokova, Magdalena
AU - Volkovova, Katarina
AU - Staruchova, Marta
AU - Hoffmann, Per
AU - Nöthen, Markus M.
AU - Dušinská, Maria
AU - Musak, Ludovit
AU - Vodicka, Pavel
AU - Hemminki, Kari
AU - Försti, Asta
PY - 2018/10/3
Y1 - 2018/10/3
N2 - Chromosomal aberrations (CAs) in human peripheral blood lymphocytes (PBL) measured with the conventional cytogenetic assay have been used for human biomonitoring of genotoxic exposure for decades. CA frequency in peripheral blood is a marker of cancer susceptibility. Previous studies have shown associations between genetic variants in metabolic pathway, DNA repair and major mitotic checkpoint genes and CAs. We conducted a genome-wide association study on 576 individuals from the Czech Republic and Slovakia followed by a replication in two different sample sets of 482 (replication 1) and 1288 (replication 2) samples. To have a broad look at the genetic susceptibility associated with CA frequency, the sample sets composed of individuals either differentially exposed to smoking, occupational/environmental hazards, or they were untreated cancer patients. Phenotypes were divided into chromosome- and chromatid-type aberrations (CSAs and CTAs, respectively) and total chromosomal aberrations (CAtot). The arbitrary cutoff point between individuals with high and low CA frequency was 2% for CAtot and 1% for CSA and CTA. The data were analyzed using age, sex, occupation/cancer and smoking history as covariates. Altogether 11 loci reached the P-value of 10−5 in the GWAS. Replication 1 supported the association of rs1383997 (8q13.3) and rs2824215 (21q21.1) in CAtot and rs983889 (5p15.1) in CTA analysis. These loci were found to be associated with genes involved in mitosis, response to environmental and chemical factors and genes involved in syndromes linked to chromosomal abnormalities. Identification of new genetic variants for the frequency of CAs offers prediction tools for cancer risk in future. 2018 Wiley Periodicals, Inc.
AB - Chromosomal aberrations (CAs) in human peripheral blood lymphocytes (PBL) measured with the conventional cytogenetic assay have been used for human biomonitoring of genotoxic exposure for decades. CA frequency in peripheral blood is a marker of cancer susceptibility. Previous studies have shown associations between genetic variants in metabolic pathway, DNA repair and major mitotic checkpoint genes and CAs. We conducted a genome-wide association study on 576 individuals from the Czech Republic and Slovakia followed by a replication in two different sample sets of 482 (replication 1) and 1288 (replication 2) samples. To have a broad look at the genetic susceptibility associated with CA frequency, the sample sets composed of individuals either differentially exposed to smoking, occupational/environmental hazards, or they were untreated cancer patients. Phenotypes were divided into chromosome- and chromatid-type aberrations (CSAs and CTAs, respectively) and total chromosomal aberrations (CAtot). The arbitrary cutoff point between individuals with high and low CA frequency was 2% for CAtot and 1% for CSA and CTA. The data were analyzed using age, sex, occupation/cancer and smoking history as covariates. Altogether 11 loci reached the P-value of 10−5 in the GWAS. Replication 1 supported the association of rs1383997 (8q13.3) and rs2824215 (21q21.1) in CAtot and rs983889 (5p15.1) in CTA analysis. These loci were found to be associated with genes involved in mitosis, response to environmental and chemical factors and genes involved in syndromes linked to chromosomal abnormalities. Identification of new genetic variants for the frequency of CAs offers prediction tools for cancer risk in future. 2018 Wiley Periodicals, Inc.
KW - chromatid-type aberrations
KW - chromosome-type aberrations
KW - GWAS
KW - single-nucleotide polymorphism
UR - http://www.scopus.com/inward/record.url?scp=85055711280&partnerID=8YFLogxK
U2 - 10.1002/em.22236
DO - 10.1002/em.22236
M3 - Article
C2 - 30368896
AN - SCOPUS:85055711280
SN - 0893-6692
JO - Environmental and Molecular Mutagenesis
JF - Environmental and Molecular Mutagenesis
ER -
