Abstract
Glioblastoma multiforme (GBM) is the most malignant and also the most common form of brain tumour in humans. The overall aim of this thesis was to investigate morphological and diagnostics features in human GBM that may be of importance for prognosis and treatment.
Immunohistochemically stained tissue sections have been microscopically analysed regarding immuno-reactive cells, cell proliferation, telomerase activity and presence of coxsackie adenovirus receptor (CAR). While telomerase activity reflects inherent life prolongation, CAR act as adhesion molecules on target cells in adenoviral based gene therapy.
We found that cytotoxic T-lymfocytes (CTL) occurred spontaneously in GBM but also that a transient increase was detected after immunotherapy. Macrophage positive cells were in general plentiful.
GBM as well as other grades of gliomas, expressed low levels of CAR while neuroblastomas and medulloblastomas exhibited a high CAR expression. However, various regions and cell types of the normal brain also expressed CAR.
Proliferation activity (Ki-67) showed a significant correlation to longer survival if index was below 10 %, while telomerase activity (hTERT) showed no relation to length of survival. Different methods of assessment of Ki-67 are of relevance for histopathological diagnostics.
This project indicates that morphological features of importance for prognosis and treatment can be identified by the use of established and simple, reliable methods, routinely applied in clinical pathological diagnostics.
Immunohistochemically stained tissue sections have been microscopically analysed regarding immuno-reactive cells, cell proliferation, telomerase activity and presence of coxsackie adenovirus receptor (CAR). While telomerase activity reflects inherent life prolongation, CAR act as adhesion molecules on target cells in adenoviral based gene therapy.
We found that cytotoxic T-lymfocytes (CTL) occurred spontaneously in GBM but also that a transient increase was detected after immunotherapy. Macrophage positive cells were in general plentiful.
GBM as well as other grades of gliomas, expressed low levels of CAR while neuroblastomas and medulloblastomas exhibited a high CAR expression. However, various regions and cell types of the normal brain also expressed CAR.
Proliferation activity (Ki-67) showed a significant correlation to longer survival if index was below 10 %, while telomerase activity (hTERT) showed no relation to length of survival. Different methods of assessment of Ki-67 are of relevance for histopathological diagnostics.
This project indicates that morphological features of importance for prognosis and treatment can be identified by the use of established and simple, reliable methods, routinely applied in clinical pathological diagnostics.
| Original language | English |
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| Qualification | Doctor |
| Awarding Institution |
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| Supervisors/Advisors |
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| Award date | 2007 May 16 |
| Publisher | |
| ISBN (Print) | 978-91-85559-62-6 |
| Publication status | Published - 2007 |
Bibliographical note
Defence detailsDate: 2007-05-16
Time: 13:00
Place: the Lecture Hall Department of Pathology Lund University Hospital
External reviewer(s)
Name: Nordborg, Claes
Title: Associate professor
Affiliation: Göteborg
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<div class="article_info">A Persson, G Skagerberg, L.G Salford and E Englund. <span class="article_issue_date">2005</span>. <span class="article_title">Immunotreatment in patients with glioblastoma multiforme – a histopathological evaluation of reactive and infl ammatory changes.</span> <span class="journal_series_title">Clinical Neuropathology</span>, <span class="journal_volume">vol 24</span> <span class="journal_pages">pp 201-208</span>.</div>
<div class="article_info">A Persson, X Fan, B Widegren and E Englund. <span class="article_issue_date">2006</span>. <span class="article_title">Cell type- and region- dependent coxsackie adenovirus receptor expression in the central nervous system.</span> <span class="journal_series_title">Journal of Neuro-Oncology</span>, <span class="journal_volume">vol 78</span> <span class="journal_pages">pp 1-6</span>.</div>
<div class="article_info">A Persson, X Fan, LG Salford, B Widegren and E Englund. <span class="article_issue_date">2007</span>. <span class="article_title">Neuroblastomas and medulloblastomas exhibit more coxsackie adenovirus receptor expression than gliomas and other brain tumours.</span> <span class="journal_series_title">Neuropathology</span>, (inpress)</div>
<div class="article_info">A Persson and E Englund. <span class="article_issue_date"></span>. <span class="article_title">Immunohistochemically stained Ki-67 but not hTERT relates to survival prognosis in glioblastoma multiforme.</span> (submitted)</div>
The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Pathology, (Lund) (013030000)
Subject classification (UKÄ)
- Cancer and Oncology
Free keywords
- General pathology
- cytogenetik
- Genetik
- Genetics
- cytogenetics
- Medicin (människa och djur)
- macrophages
- adenoviral based gene-therapy proliferation activity
- telomerase activity
- immunohistochemistry
- Medicine (human and vertebrates)
- cytotoxic T-lymphocytes
- diagnostic features
- prognosis
- immuno-therapy
- Glioblastoma multiforme
- neuropathology
- pathological anatomy
- Patologi (allmän)
- patologisk anatomi
