Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis.

Shashank Gupta; Regine Utoft; Henrik Hasseldam; Anja Schmidt-Christensen; Tine Hannibal; Lisbeth Hansen; Nina Fransén Pettersson; Noopur Agarwal-Gupta; Björn Rozell; Åsa Andersson; Dan Holmberg

doi:10.1371/journal.pone.0076330

Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis.

Shashank Gupta, Regine Utoft, Henrik Hasseldam, Anja Schmidt-Christensen, Tine Hannibal, Lisbeth Hansen, Nina Fransén Pettersson, Noopur Agarwal-Gupta, Björn Rozell, Åsa Andersson, Dan Holmberg

Department of Experimental Medical Science

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Abstract

Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.

Original language	English
Article number	e76330
Journal	PLoS ONE
Volume	8
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0076330
Publication status	Published - 2013

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Department of Experimental Medical Science (013210000), Autoimmunity (013210023), Medical Inflammation Research (013212019)

Subject classification (UKÄ)

Basic Medicine

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10.1371/journal.pone.0076330

PLoS__Gupta_et_al

http://www.ncbi.nlm.nih.gov/pubmed/24124545?dopt=Abstract

3D imaging of inflammation and autoimmunity
Schmidt-Christensen, A. (Researcher), Holmberg, D. (PI), Lasser, T. (PI) & Nguyen, D. (Research student)
2009/10/01 → 2020/07/31
Project: Research

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@article{a849f353a2ea4e1397f4158c56191c00,

title = "Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis.",

abstract = "Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.",

author = "Shashank Gupta and Regine Utoft and Henrik Hasseldam and Anja Schmidt-Christensen and Tine Hannibal and Lisbeth Hansen and {Frans{\'e}n Pettersson}, Nina and Noopur Agarwal-Gupta and Bj{\"o}rn Rozell and {\AA}sa Andersson and Dan Holmberg",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Experimental Medical Science (013210000), Autoimmunity (013210023), Medical Inflammation Research (013212019)",

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doi = "10.1371/journal.pone.0076330",

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T1 - Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis.

AU - Gupta, Shashank

AU - Utoft, Regine

AU - Hasseldam, Henrik

AU - Schmidt-Christensen, Anja

AU - Hannibal, Tine

AU - Hansen, Lisbeth

AU - Fransén Pettersson, Nina

AU - Agarwal-Gupta, Noopur

AU - Rozell, Björn

AU - Andersson, Åsa

AU - Holmberg, Dan

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Experimental Medical Science (013210000), Autoimmunity (013210023), Medical Inflammation Research (013212019)

PY - 2013

Y1 - 2013

N2 - Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.

AB - Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.

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DO - 10.1371/journal.pone.0076330

M3 - Article

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SN - 1932-6203

VL - 8

JO - PLoS ONE

JF - PLoS ONE

IS - 10

M1 - e76330

ER -

Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis.

Abstract

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3D imaging of inflammation and autoimmunity

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