Glucose transport activity measured in giant vesicles

Jesper S. Hansen, Karin Lindkvist-Petersson

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages77-91
Number of pages15
Volume1713
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1713
ISSN (Print)1064-3745

Subject classification (UKÄ)

  • Cell and Molecular Biology

Keywords

  • Activity assay
  • Fluorescence microscopy
  • Giant vesicles
  • Glucose transport
  • Glucose transporters
  • GLUT1
  • Membrane protein
  • Resorufin

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