Glucose transport activity measured in giant vesicles

Jesper S. Hansen, Karin Lindkvist-Petersson

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review


Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Number of pages15
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Subject classification (UKÄ)

  • Cell and Molecular Biology


  • Activity assay
  • Fluorescence microscopy
  • Giant vesicles
  • Glucose transport
  • Glucose transporters
  • GLUT1
  • Membrane protein
  • Resorufin


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