HASH-1 and E2-2 are expressed in human neuroblastoma cells and form a functional complex

Paula Persson, Annika Jögi, Anna Grynfeld, Sven Påhlman, Håkan Axelson

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)


The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homolog-1 (MASH-1 in mouse and HASH-1 in human) is essential for proper development of olfactory and most peripheral autonomic neurons, and for the formation of distinct neuronal circuits within the central nervous system. We have previously shown that HASH-1 is expressed in neuroblastoma tumors and cell lines, and in this study we have used the yeast two-hybrid system to isolate HASH-1 interacting proteins from a human neuroblastoma cDNA library. Two of the isolated clones contained cDNA from the E2-2 gene (also known as ITF2/SEF2-1). We show that E2-2 interacts with HASH-1 in both yeast and mammalian cells. The HASH-1/E2-2 complex binds an E-box (CACCTG) in vitro, and transactivates an E-box containing reporter construct in vivo. Furthermore, E2-2 seems to be one of the major HASH-1 interacting proteins in extracts from neuroblastoma cells. In conclusion, E2-2 forms a functional complex with HASH-1, and might therefore be involved in the development of specific parts of the central and peripheral nervous systems.
Original languageEnglish
Pages (from-to)22-31
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 2000

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Molecular Medicine (013031200), Molecular Tumour Biology (013017540), Division of Molecular Medicine and Gene Therapy (013022010)

Subject classification (UKÄ)

  • Biological Sciences


  • SEF2-1
  • neuroblastoma
  • HASH-1
  • sympathetic nervous system
  • E2-2
  • bHLH
  • differentiation
  • yeast two-hybrid


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