TY - JOUR
T1 - Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study
AU - Myhrstad, Mari C.W.
AU - de Mello, Vanessa D.
AU - Dahlman, Ingrid
AU - Kolehmainen, Marjukka
AU - Paananen, Jussi
AU - Rundblad, Amanda
AU - Carlberg, Carsten
AU - Olstad, Ole Kristoffer
AU - Pihlajamäki, Jussi
AU - Holven, Kirsten B.
AU - Hermansen, Kjeld
AU - Dragsted, Lars O.
AU - Gunnarsdottir, Ingibjörg
AU - Cloetens, Lieselotte
AU - Storm, Matilda Ulmius
AU - Åkesson, Björn
AU - Rosqvist, Fredrik
AU - Hukkanen, Janne
AU - Herzig, Karl Heinz
AU - Risérus, Ulf
AU - Thorsdottir, Inga
AU - Poutanen, Kaisa S.
AU - Savolainen, Markku J.
AU - Schwab, Ursula
AU - Arner, Peter
AU - Uusitupa, Matti
AU - Ulven, Stine M.
PY - 2019/7
Y1 - 2019/7
N2 - Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.
AB - Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.
KW - gene-expression
KW - healthy Nordic diet
KW - metabolic syndrome
KW - peripheral blood mononuclear cells
KW - transcriptome
U2 - 10.1002/mnfr.201801405
DO - 10.1002/mnfr.201801405
M3 - Article
C2 - 30964598
AN - SCOPUS:85064710489
SN - 1613-4125
VL - 63
JO - Molecular Nutrition & Food Research
JF - Molecular Nutrition & Food Research
IS - 13
M1 - 1801405
ER -