Helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst

Sujinna Lekmeechai; Yu Ching Su; Marta Brant; Maria Alvarado-Kristensson; Anna Vallström; Ikenna Obi; Anna Arnqvist; Kristian Riesbeck

doi:10.3389/fmicb.2018.01837

Helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst

Sujinna Lekmeechai, Yu Ching Su, Marta Brant, Maria Alvarado-Kristensson, Anna Vallström, Ikenna Obi, Anna Arnqvist, Kristian Riesbeck

Umeå University

Research output: Contribution to journal › Article › peer-review

Abstract

Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H₂O₂ and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion.

Original language	English
Article number	1837
Journal	Frontiers in Microbiology
Volume	9
Issue number	SEP
DOIs	https://doi.org/10.3389/fmicb.2018.01837
Publication status	Published - 2018 Sept 7

Subject classification (UKÄ)

Microbiology in the Medical Area

Free keywords

H. pylori
KatA
Outer membrane vesicles
Oxidative burst
Reactive oxygen species

Access to Document

10.3389/fmicb.2018.01837

Cite this

@article{55a66bb917ca4e7ab4cf510f7e3c4f8c,

title = "Helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst",

abstract = "Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H2O2 and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion.",

keywords = "H. pylori, KatA, Outer membrane vesicles, Oxidative burst, Reactive oxygen species",

author = "Sujinna Lekmeechai and Su, {Yu Ching} and Marta Brant and Maria Alvarado-Kristensson and Anna Vallstr{\"o}m and Ikenna Obi and Anna Arnqvist and Kristian Riesbeck",

year = "2018",

month = sep,

day = "7",

doi = "10.3389/fmicb.2018.01837",

language = "English",

volume = "9",

journal = "Frontiers in Microbiology",

issn = "1664-302X",

publisher = "Frontiers Media S. A.",

number = "SEP",

}

TY - JOUR

T1 - Helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst

AU - Lekmeechai, Sujinna

AU - Su, Yu Ching

AU - Brant, Marta

AU - Alvarado-Kristensson, Maria

AU - Vallström, Anna

AU - Obi, Ikenna

AU - Arnqvist, Anna

AU - Riesbeck, Kristian

PY - 2018/9/7

Y1 - 2018/9/7

N2 - Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H2O2 and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion.

AB - Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H2O2 and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion.

KW - H. pylori

KW - KatA

KW - Outer membrane vesicles

KW - Oxidative burst

KW - Reactive oxygen species

U2 - 10.3389/fmicb.2018.01837

DO - 10.3389/fmicb.2018.01837

M3 - Article

C2 - 30245670

AN - SCOPUS:85053053080

SN - 1664-302X

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

IS - SEP

M1 - 1837

ER -

Helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst

Abstract

Subject classification (UKÄ)

Free keywords

Access to Document

Fingerprint

Cite this