High GAD65 autoantibody levels in nondiabetic adults are associated with HLA but not with CTLA-4 or INS VNTR.

O Rolandsson, E Hägg, M Janer, E Rutledge, L K Gaur, M Nilsson, G Hallmans, Åke Lernmark

Research output: Contribution to journalArticlepeer-review

13 Citations (SciVal)

Abstract

OBJECTIVES: To explore the relationship between genetic background and antibody levels in a nondiabetic population. We evaluated if high levels of autoantibodies against the 65 kDa isoform of glutamic acid decarboxylase (GAD65Ab), were associated with high-risk genes, i.e. HLA, CTLA-4 and INS VNTR genes. DESIGN AND SUBJECTS: Seventy-five (M/F 39/36) subjects exceeding the 95th percentile of GAD65 autoantibody index and 75 age and sex matched subjects below the 95th percentile, randomly selected amongst participants in the Västerbotten Intervention Programme. METHODS: The GAD65 Ab were measured in a radioligand-binding assay. HLA class II typing was performed by an oligoblot hybridization method. CTLA-4 repeat length was analysed and divided into short forms and long forms. Class I and class III alleles of INS VNTR were detected. Differences in distribution were tested by Pearson chi-square with Yates correction. Odds ratios (OR) were used to compare groups calculated with Cochran's and
Original languageEnglish
Pages (from-to)447-453
JournalJournal of Internal Medicine
Volume253
Issue number4
DOIs
Publication statusPublished - 2003

Subject classification (UKÄ)

  • Endocrinology and Diabetes

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