Abstract
In microbiology, cell analysis is an important tool for understanding influences on biotechnological production. Using current bulk methods, the secretome cannot be assigned to the corresponding cell. This contribution reports a novel method for cell separation inside microscale droplets. Cells are focused in droplets using bulk acoustic waves before the droplet is split at a bifurcation. For a wide range of experimental parameters, an average separation efficiency of above 99% is achieved. The usability of the device for yeast cells is evaluated. This work demonstrates a missing key element for future high-throughput single-cell analysis techniques based on mass spectrometry.
| Original language | English |
|---|---|
| Title of host publication | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 902-903 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419000 |
| Publication status | Published - 2019 |
| Externally published | Yes |
| Event | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland Duration: 2019 Oct 27 → 2019 Oct 31 |
Publication series
| Name | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
|---|
Conference
| Conference | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
|---|---|
| Country/Territory | Switzerland |
| City | Basel |
| Period | 2019/10/27 → 2019/10/31 |
Bibliographical note
Publisher Copyright:© 2019 CBMS-0001.
Subject classification (UKÄ)
- Biomedical Laboratory Science/Technology
Free keywords
- Cell manipulation
- Cell secretome separation
- Microfluidics
- Microscale droplets