High-throughput identification of genes promoting neuron formation and lineage choice in mouse embryonic stem cells

Anna Falk, Tobias E Karlsson, Sanja Kurdija, Jonas Frisén, Joel Zupicich

Research output: Contribution to journalArticlepeer-review

Abstract

The potential of embryonic stem cells to differentiate to all cell types makes them an attractive model for development and a potential source of cells for transplantation therapies. Candidate approaches have identified individual genes and proteins that promote the differentiation of embryonic stem cells to desired fates. Here, we describe a rapid large-scale screening strategy for the identification of genes that influence the pluripotency and differentiation of embryonic stem cells to specific fates, and we use this approach to identify genes that induce neuron formation. The power of the strategy is validated by the fact that, of the 15 genes that resulted in the largest increase in neuron number, 8 have previously been implicated in neuronal differentiation or survival, whereas 7 represent novel genes or known genes not previously implicated in neuronal development. This is a simple, fast, and generally applicable strategy for the identification of genes promoting the formation of any specific cell type from embryonic stem cells. Disclosure of potential conflicts of interest is found at the end of this article.

Original languageEnglish
Pages (from-to)1539-45
Number of pages7
JournalStem Cells
Volume25
Issue number6
DOIs
Publication statusPublished - 2007 Jun
Externally publishedYes

Subject classification (UKÄ)

  • Neurosciences
  • Cell and Molecular Biology

Keywords

  • Animals
  • Cell Differentiation/genetics
  • Cell Lineage/genetics
  • Cells, Cultured
  • Embryonic Stem Cells/cytology
  • Gene Expression Profiling/methods
  • Genes, Developmental
  • Mice
  • Models, Biological
  • Neurons/cytology
  • Organ Specificity/genetics
  • Transfection/methods

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