HPV16 E5 is produced from an HPV16 early mRNA spliced from SD226 to SA3358

Kersti Nilsson; Christopher Norberg; Ann-Kristin Mossberg; Stefan Schwartz

doi:10.1016/j.virusres.2017.11.009

HPV16 E5 is produced from an HPV16 early mRNA spliced from SD226 to SA3358

Kersti Nilsson, Christopher Norberg, Ann-Kristin Mossberg, Stefan Schwartz

Lund University

Research output: Contribution to journal › Article › peer-review

Abstract

The HPV16 E5 open reading frame (ORF) is present on the majority of all alternatively spliced HPV16 mRNAs, but it is currently unknown how well it is translated into E5 protein. To identify HPV16 mRNAs that are efficiently translated into E5, we have generated cDNA plasmids expressing individual, alternatively spliced HPV16 mRNAs with the potential to produce E5. By replacing the E5 ORF with sLuc, we could quantitate sLuc and determine how well each cDNA was translated. Our results showed that the upstream E1 and E7 AUGs inhibited translation of the E5 ORF and revealed that only one HPV16 mRNA produced high levels of E5. This was an HPV16 early mRNA spliced from SD226 to SA3358. These results were confirmed in the context of the entire HPV16 genome. Taken together, our results indicate that E5 is expressed early in the HPV16 replication cycle since it is translated efficiently only by one early mRNA.

Original language	English
Pages (from-to)	128-136
Journal	Virus Research
Volume	244
Early online date	2017
DOIs	https://doi.org/10.1016/j.virusres.2017.11.009
Publication status	Published - 2018

Subject classification (UKÄ)

Microbiology in the medical area

Keywords

E5
Hpv
mRNA
Splicing
Translation

Access to Document

10.1016/j.virusres.2017.11.009

Cite this

@article{de5b22be835447d38c66417f511ff0ab,

title = "HPV16 E5 is produced from an HPV16 early mRNA spliced from SD226 to SA3358",

abstract = "The HPV16 E5 open reading frame (ORF) is present on the majority of all alternatively spliced HPV16 mRNAs, but it is currently unknown how well it is translated into E5 protein. To identify HPV16 mRNAs that are efficiently translated into E5, we have generated cDNA plasmids expressing individual, alternatively spliced HPV16 mRNAs with the potential to produce E5. By replacing the E5 ORF with sLuc, we could quantitate sLuc and determine how well each cDNA was translated. Our results showed that the upstream E1 and E7 AUGs inhibited translation of the E5 ORF and revealed that only one HPV16 mRNA produced high levels of E5. This was an HPV16 early mRNA spliced from SD226 to SA3358. These results were confirmed in the context of the entire HPV16 genome. Taken together, our results indicate that E5 is expressed early in the HPV16 replication cycle since it is translated efficiently only by one early mRNA.",

keywords = "E5, Hpv, mRNA, Splicing, Translation",

author = "Kersti Nilsson and Christopher Norberg and Ann-Kristin Mossberg and Stefan Schwartz",

year = "2018",

doi = "10.1016/j.virusres.2017.11.009",

language = "English",

volume = "244",

pages = "128--136",

journal = "Virus Research",

issn = "1872-7492",

publisher = "Elsevier",

}

TY - JOUR

T1 - HPV16 E5 is produced from an HPV16 early mRNA spliced from SD226 to SA3358

AU - Nilsson, Kersti

AU - Norberg, Christopher

AU - Mossberg, Ann-Kristin

AU - Schwartz, Stefan

PY - 2018

Y1 - 2018

N2 - The HPV16 E5 open reading frame (ORF) is present on the majority of all alternatively spliced HPV16 mRNAs, but it is currently unknown how well it is translated into E5 protein. To identify HPV16 mRNAs that are efficiently translated into E5, we have generated cDNA plasmids expressing individual, alternatively spliced HPV16 mRNAs with the potential to produce E5. By replacing the E5 ORF with sLuc, we could quantitate sLuc and determine how well each cDNA was translated. Our results showed that the upstream E1 and E7 AUGs inhibited translation of the E5 ORF and revealed that only one HPV16 mRNA produced high levels of E5. This was an HPV16 early mRNA spliced from SD226 to SA3358. These results were confirmed in the context of the entire HPV16 genome. Taken together, our results indicate that E5 is expressed early in the HPV16 replication cycle since it is translated efficiently only by one early mRNA.

AB - The HPV16 E5 open reading frame (ORF) is present on the majority of all alternatively spliced HPV16 mRNAs, but it is currently unknown how well it is translated into E5 protein. To identify HPV16 mRNAs that are efficiently translated into E5, we have generated cDNA plasmids expressing individual, alternatively spliced HPV16 mRNAs with the potential to produce E5. By replacing the E5 ORF with sLuc, we could quantitate sLuc and determine how well each cDNA was translated. Our results showed that the upstream E1 and E7 AUGs inhibited translation of the E5 ORF and revealed that only one HPV16 mRNA produced high levels of E5. This was an HPV16 early mRNA spliced from SD226 to SA3358. These results were confirmed in the context of the entire HPV16 genome. Taken together, our results indicate that E5 is expressed early in the HPV16 replication cycle since it is translated efficiently only by one early mRNA.

KW - E5

KW - Hpv

KW - mRNA

KW - Splicing

KW - Translation

UR - http://www.scopus.com/inward/record.url?scp=85034420391&partnerID=8YFLogxK

U2 - 10.1016/j.virusres.2017.11.009

DO - 10.1016/j.virusres.2017.11.009

M3 - Article

C2 - 29155138

AN - SCOPUS:85034420391

VL - 244

SP - 128

EP - 136

JO - Virus Research

JF - Virus Research

SN - 1872-7492

ER -

HPV16 E5 is produced from an HPV16 early mRNA spliced from SD226 to SA3358

Abstract

Subject classification (UKÄ)

Keywords

Access to Document

Other files and links

Fingerprint

The role of cellular RNA processing functions in Human papillomavirus type 16 gene regulation

Cite this