Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Christa Broholm, Anders Henrik Olsson, Alexander Perfilyev, Linn Gillberg, Ninna Schiøler Hansen, Ashfaq Ali, Brynjulf Mortensen, Charlotte Ling, Allan Vaag

Research output: Contribution to journalArticlepeer-review

14 Citations (SciVal)

Abstract

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

Original languageEnglish
Pages (from-to)1601-1617
Number of pages17
JournalEpigenomics
Volume8
Issue number12
DOIs
Publication statusPublished - 2016 Dec 1

Subject classification (UKÄ)

  • Endocrinology and Diabetes
  • Medical Genetics

Keywords

  • adipogenesis
  • adiponectin
  • DNA methylation
  • epigenetics
  • stem cells

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