Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction

Mikael Adner; David Erlinge; Leif Salford; Frances Yee; Claes Wahlestedt; Lars Edvinsson

doi:10.1016/0014-2999(94)90118-X

Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction

Mikael Adner, David Erlinge, Leif Salford, Frances Yee, Claes Wahlestedt, Lars Edvinsson

Research output: Contribution to journal › Article › peer-review

Abstract

Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery.

Original language	English
Pages (from-to)	281-284
Journal	European Journal of Pharmacology
Volume	261
Issue number	3
DOIs	https://doi.org/10.1016/0014-2999(94)90118-X
Publication status	Published - 1994

Subject classification (UKÄ)

Pharmacology and Toxicology

Free keywords

Antisense oligodeoxynucleotide
Endothelin ETA receptor
Temporal artery
human

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10.1016/0014-2999(94)90118-X

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title = "Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction",

abstract = "Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery.",

keywords = "Antisense oligodeoxynucleotide, Endothelin ETA receptor, Temporal artery, human",

author = "Mikael Adner and David Erlinge and Leif Salford and Frances Yee and Claes Wahlestedt and Lars Edvinsson",

year = "1994",

doi = "10.1016/0014-2999(94)90118-X",

language = "English",

volume = "261",

pages = "281--284",

journal = "European Journal of Pharmacology",

issn = "1879-0712",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction

AU - Adner, Mikael

AU - Erlinge, David

AU - Salford, Leif

AU - Yee, Frances

AU - Wahlestedt, Claes

AU - Edvinsson, Lars

PY - 1994

Y1 - 1994

N2 - Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery.

AB - Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery.

KW - Antisense oligodeoxynucleotide

KW - Endothelin ETA receptor

KW - Temporal artery

KW - human

U2 - 10.1016/0014-2999(94)90118-X

DO - 10.1016/0014-2999(94)90118-X

M3 - Article

SN - 1879-0712

VL - 261

SP - 281

EP - 284

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 3

ER -

Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction

Abstract

Subject classification (UKÄ)

Free keywords

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