Glyceryl monooleate (GMO) and other unsaturated long-chain monoglycerides show peculiar phase behaviour characterised by the presence of one or more cubic liquid-crystalline phases. In the present study, hydrolysis of cubic lipid-water phases formed by GMO were determined. Human pancreatic carboxyl ester lipase (CEL), colipase-dependent lipase with colipase, human duodenal contents and rat pancreas homogenate were added and the release of free oleic acid from GMO was determined. It was found that pancreatic enzymes hydrolyse GMO cubic lipid-water phases in vitro. In the presence of concentrations of sodium taurocholate, sodium taourodeoxycholate or sodium glycocholate, above 3 mM, the hydrolysis by pancreatic enzymes was stimulated. The optimal pH for the hydrolysis of GMO by CEL was 7.5-8.5, by colipase-dependent lipase 6-9 with a peak at 6.5 and by rat pancreas homogenate 6-8.5 with a peak at 7.3. The hydrolysis of GMO with human duodenal content was slow but increased with time. These results indicated that pancreatic enzymes hydrolyse GMO in cubic lipid-water phases. The hydrolysis is effected by pH and the concentration of bile salts.
|Conference||Conference on Lipid and Polymer-Lipid Systems, 2002|
|Period||2002/10/01 → 2002/10/02|
- glyceryl monooleate
- cubic liquid-crystalline phase