Hydrolysis of the cubic liquid-crystalline phase of glyceryl monooleate by human pancreatic lipases

Li Zhou, T Landh, Berit Sternby, Åke Nilsson

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceedingResearchpeer-review

6 Citations (Scopus)


Glyceryl monooleate (GMO) and other unsaturated long-chain monoglycerides show peculiar phase behaviour characterised by the presence of one or more cubic liquid-crystalline phases. In the present study, hydrolysis of cubic lipid-water phases formed by GMO were determined. Human pancreatic carboxyl ester lipase (CEL), colipase-dependent lipase with colipase, human duodenal contents and rat pancreas homogenate were added and the release of free oleic acid from GMO was determined. It was found that pancreatic enzymes hydrolyse GMO cubic lipid-water phases in vitro. In the presence of concentrations of sodium taurocholate, sodium taourodeoxycholate or sodium glycocholate, above 3 mM, the hydrolysis by pancreatic enzymes was stimulated. The optimal pH for the hydrolysis of GMO by CEL was 7.5-8.5, by colipase-dependent lipase 6-9 with a peak at 6.5 and by rat pancreas homogenate 6-8.5 with a peak at 7.3. The hydrolysis of GMO with human duodenal content was slow but increased with time. These results indicated that pancreatic enzymes hydrolyse GMO in cubic lipid-water phases. The hydrolysis is effected by pH and the concentration of bile salts.
Original languageEnglish
Title of host publicationLipid and Polymer-Lipid Systems (Progress in Colloid and Polymer Science)
ISBN (Print)978-3-540-43001-8
Publication statusPublished - 2002
EventConference on Lipid and Polymer-Lipid Systems, 2002 - Chia Laguna, Italy
Duration: 2002 Oct 12002 Oct 2

Publication series

ISSN (Print)0340-255X


ConferenceConference on Lipid and Polymer-Lipid Systems, 2002
CityChia Laguna

Subject classification (UKÄ)

  • Other Clinical Medicine


  • bile
  • hydrolysis
  • glyceryl monooleate
  • cubic liquid-crystalline phase
  • lipases
  • salt


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