Hypothalamic gene expression following ghrelin therapy to gastrectomized rodents

Emil Egecioglu; Bjorn Stenstrom; Scarlett B Pinnock; Loraine Y C Tung; Charlotta de la Cour; Andreas Lindqvist; Rolf Håkanson; Unni Syversen; Duan Chen; Suzanne L Dickson

doi:10.1016/j.regpep.2007.09.006

Hypothalamic gene expression following ghrelin therapy to gastrectomized rodents

Emil Egecioglu, Bjorn Stenstrom, Scarlett B Pinnock, Loraine Y C Tung, Charlotta de la Cour, Andreas Lindqvist, Rolf Håkanson, Unni Syversen, Duan Chen, Suzanne L Dickson

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated whether ghrelin depletion (by gastrectomy surgery) and/or treatment/replacement with the gastric hormone ghrelin alters the expression of key hypothalamic genes involved in energy balance, in a manner consistent with ghrelin's pro-obesity effects. At 2 weeks after surgery mice were treated with ghrelin (12 nmol/mouse/day, sc) or vehicle for 8 weeks. Gastrectomy had little effect on the expression of these genes, with the exception of NPY mRNA in the arcuate nucleus that was increased. Ghrelin treatment (to gastrectomized and sham mice) increased the mRNA expression of orexigenic peptides NPY and AgRP while decreasing mRNA expression of the anorexigenic peptide POMC. Two weeks gavage treatment with the ghrelin mimetic, MK-0677, to rats increased NPY and POMC mRNA in the arcuate nucleus and MCH mRNA in the lateral hypothalamus. Thus, while predicted pro-obesity ghrelin signalling pathways were activated by ghrelin and ghrelin mimetics, these were largely unaffected by gastrectomy.

Original language	English
Pages (from-to)	176-182
Journal	Regulatory Peptides
Volume	146
Issue number	1-3
DOIs	https://doi.org/10.1016/j.regpep.2007.09.006
Publication status	Published - 2008

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10.1016/j.regpep.2007.09.006

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title = "Hypothalamic gene expression following ghrelin therapy to gastrectomized rodents",

abstract = "We investigated whether ghrelin depletion (by gastrectomy surgery) and/or treatment/replacement with the gastric hormone ghrelin alters the expression of key hypothalamic genes involved in energy balance, in a manner consistent with ghrelin's pro-obesity effects. At 2 weeks after surgery mice were treated with ghrelin (12 nmol/mouse/day, sc) or vehicle for 8 weeks. Gastrectomy had little effect on the expression of these genes, with the exception of NPY mRNA in the arcuate nucleus that was increased. Ghrelin treatment (to gastrectomized and sham mice) increased the mRNA expression of orexigenic peptides NPY and AgRP while decreasing mRNA expression of the anorexigenic peptide POMC. Two weeks gavage treatment with the ghrelin mimetic, MK-0677, to rats increased NPY and POMC mRNA in the arcuate nucleus and MCH mRNA in the lateral hypothalamus. Thus, while predicted pro-obesity ghrelin signalling pathways were activated by ghrelin and ghrelin mimetics, these were largely unaffected by gastrectomy.",

author = "Emil Egecioglu and Bjorn Stenstrom and Pinnock, \{Scarlett B\} and Tung, \{Loraine Y C\} and \{de la Cour\}, Charlotta and Andreas Lindqvist and Rolf H{\aa}kanson and Unni Syversen and Duan Chen and Dickson, \{Suzanne L\}",

year = "2008",

doi = "10.1016/j.regpep.2007.09.006",

language = "English",

volume = "146",

pages = "176--182",

journal = "Regulatory Peptides",

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T1 - Hypothalamic gene expression following ghrelin therapy to gastrectomized rodents

AU - Egecioglu, Emil

AU - Stenstrom, Bjorn

AU - Pinnock, Scarlett B

AU - Tung, Loraine Y C

AU - de la Cour, Charlotta

AU - Lindqvist, Andreas

AU - Håkanson, Rolf

AU - Syversen, Unni

AU - Chen, Duan

AU - Dickson, Suzanne L

PY - 2008

Y1 - 2008

N2 - We investigated whether ghrelin depletion (by gastrectomy surgery) and/or treatment/replacement with the gastric hormone ghrelin alters the expression of key hypothalamic genes involved in energy balance, in a manner consistent with ghrelin's pro-obesity effects. At 2 weeks after surgery mice were treated with ghrelin (12 nmol/mouse/day, sc) or vehicle for 8 weeks. Gastrectomy had little effect on the expression of these genes, with the exception of NPY mRNA in the arcuate nucleus that was increased. Ghrelin treatment (to gastrectomized and sham mice) increased the mRNA expression of orexigenic peptides NPY and AgRP while decreasing mRNA expression of the anorexigenic peptide POMC. Two weeks gavage treatment with the ghrelin mimetic, MK-0677, to rats increased NPY and POMC mRNA in the arcuate nucleus and MCH mRNA in the lateral hypothalamus. Thus, while predicted pro-obesity ghrelin signalling pathways were activated by ghrelin and ghrelin mimetics, these were largely unaffected by gastrectomy.

AB - We investigated whether ghrelin depletion (by gastrectomy surgery) and/or treatment/replacement with the gastric hormone ghrelin alters the expression of key hypothalamic genes involved in energy balance, in a manner consistent with ghrelin's pro-obesity effects. At 2 weeks after surgery mice were treated with ghrelin (12 nmol/mouse/day, sc) or vehicle for 8 weeks. Gastrectomy had little effect on the expression of these genes, with the exception of NPY mRNA in the arcuate nucleus that was increased. Ghrelin treatment (to gastrectomized and sham mice) increased the mRNA expression of orexigenic peptides NPY and AgRP while decreasing mRNA expression of the anorexigenic peptide POMC. Two weeks gavage treatment with the ghrelin mimetic, MK-0677, to rats increased NPY and POMC mRNA in the arcuate nucleus and MCH mRNA in the lateral hypothalamus. Thus, while predicted pro-obesity ghrelin signalling pathways were activated by ghrelin and ghrelin mimetics, these were largely unaffected by gastrectomy.

UR - https://www.scopus.com/pages/publications/38149059686

U2 - 10.1016/j.regpep.2007.09.006

DO - 10.1016/j.regpep.2007.09.006

M3 - Article

SN - 1873-1686

VL - 146

SP - 176

EP - 182

JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 1-3

ER -

Hypothalamic gene expression following ghrelin therapy to gastrectomized rodents

Abstract

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