Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

Katarzyna M. Luda; Clement Da Silva; Fatemeh Ahmadi; Allan Mcl Mowat; Hiroshi Ohno; Knut Kotarsky; William W. Agace

doi:10.1111/sji.13219

Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

Katarzyna M. Luda, Clement Da Silva, Fatemeh Ahmadi, Allan Mcl Mowat, Hiroshi Ohno, Knut Kotarsky, William W. Agace

Research output: Contribution to journal › Article › peer-review

Abstract

The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103⁺CD11b⁺ cDC in the murine small intestine (SI). GP2 expression was induced on CD103⁺CD11b⁺ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103⁺CD11b⁺ cDC (huLang-Cre.gp2^fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103⁺CD11b⁺ cDC is not required for intestinal immune homoeostasis.

Original language	English
Article number	e13219
Pages (from-to)	1-12
Journal	Scandinavian Journal of Immunology
Volume	96
Issue number	5
DOIs	https://doi.org/10.1111/sji.13219
Publication status	Published - 2022 Nov

Subject classification (UKÄ)

Immunology in the medical area

Keywords

cDC2
dendritic cell
homeostasis
intestine
IRF4
retinoic acid
TGFβR1

Access to Document

10.1111/sji.13219Licence: CC BY

Cite this

@article{859dfe8c562a45e0910299b10ce3e3f4,

title = "Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells",

abstract = "The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.",

keywords = "cDC2, dendritic cell, homeostasis, intestine, IRF4, retinoic acid, TGFβR1",

author = "Luda, {Katarzyna M.} and {Da Silva}, Clement and Fatemeh Ahmadi and Mowat, {Allan Mcl} and Hiroshi Ohno and Knut Kotarsky and Agace, {William W.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors. Scandinavian Journal of Immunology published by John Wiley & Sons Ltd on behalf of The Scandinavian Foundation for Immunology.",

year = "2022",

month = nov,

doi = "10.1111/sji.13219",

language = "English",

volume = "96",

pages = "1--12",

journal = "Scandinavian Journal of Immunology",

issn = "0300-9475",

publisher = "Wiley-Blackwell",

number = "5",

}

TY - JOUR

T1 - Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

AU - Luda, Katarzyna M.

AU - Da Silva, Clement

AU - Ahmadi, Fatemeh

AU - Mowat, Allan Mcl

AU - Ohno, Hiroshi

AU - Kotarsky, Knut

AU - Agace, William W.

PY - 2022/11

Y1 - 2022/11

N2 - The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.

AB - The intestinal lamina propria (LP) contains distinct subsets of classical dendritic cells (cDC), each playing key non-redundant roles in intestinal immune homeostasis. Here, we show that glycoprotein 2 (GP2), a GPI-anchored protein and receptor for bacterial type-I fimbriae, is selectively expressed by CD103+CD11b+ cDC in the murine small intestine (SI). GP2 expression was induced on CD103+CD11b+ cDC within the SI-LP and was regulated by IRF4, TGFβR1- and retinoic acid signalling. Mice selectively lacking Gp2 on CD103+CD11b+ cDC (huLang-Cre.gp2fl/fl mice) had normal numbers and proportions of innate and adaptive immune cells in the SI-LP suggesting that GP2 expression by CD103+CD11b+ cDC is not required for intestinal immune homoeostasis.

KW - cDC2

KW - dendritic cell

KW - homeostasis

KW - intestine

KW - IRF4

KW - retinoic acid

KW - TGFβR1

U2 - 10.1111/sji.13219

DO - 10.1111/sji.13219

M3 - Article

AN - SCOPUS:85140009640

VL - 96

SP - 1

EP - 12

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 5

M1 - e13219

ER -

Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells

Abstract

Subject classification (UKÄ)

Keywords

Access to Document

Fingerprint

Cite this