Identification and quantitative measurements of biogenic amines and Dopa in the central nervous system and haemolymph of the crayfish Pacifastacus leniusculus (Crustacea)

Rolf Elofsson, Lena Laxmyr, Evald Rosegren, Christer Hansson

Research output: Contribution to journalArticlepeer-review

Abstract

1. The biogenic amines dopamine (DA), noradrenaline (NA), octopamine (OA), serotonin (5-HT) and the amino acid dihydroxyphenylalanine (DOPA) have been identified and quantitatively measured in the crayfish nervous system and in haemolymph employing high performance liquid chromatography (HPLC).

2. The substances occurred in the nervous system in amounts ranging between 0.1 and 0.7 μg/g w/w.

3. The haemolymph contained very small amounts of NA, OA, 5-HT and DOPA.

4. No adrenaline (A) was present.

5. Prolonged handling of the animals caused dramatic changes in DOPA levels.

6. Depletion experiments using reserpine caused a pronounced decrease in the content of DA, NA, OA and 5-HT in the nervous system. DOPA, however, remained unaffected.
Original languageEnglish
Pages (from-to)195-201
JournalComparative Biochemistry and Physiology. Part C: Comparative Pharmacology & Toxicology
Volume71
Publication statusPublished - 1982

Subject classification (UKÄ)

  • Zoology

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