Immobilised activated sludge based biosensor for biochemical oxygen demand measurement

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A biochemical oxygen demand (BOD) sensor, based on an immobilised mixed culture of microorganisms in combination with a dissolved oxygen electrode, has been developed for the purpose of on-line monitoring of the biological treatment process for waste and wastewater. The sensor was designed for easy replacement of the biomembrane, thereby making it suitable for short-term use. The drawbacks of activated sludge based sensor, such as short sensor lifetime, were thereby circumvented. The sensor BOD measurements were carried out in the kinetic mode using a flow injection system, resulting in 25 s for one measurement followed by 4-8 min recovery time. Based on the results of normalised sensor responses, the OECD synthetic wastewater was considered to be a more suitable calibration solution in comparison with the GGA solution. Good agreement was achieved between the results of the sensor BOD measurement and those obtained from BOD5 analysis of a wastewater sample from a food-processing factory. Reproducibility of responses using one sensor was below +/-5.6% standard deviation. Reproducibility of responses using different sensors was within acceptable bias limits, viz. +/-15% standard deviation.
Original languageEnglish
Pages (from-to)883-893
JournalBiosensors & Bioelectronics
Issue number12
Publication statusPublished - 2000

Subject classification (UKÄ)

  • Industrial Biotechnology


  • Biochemical oxygen demand
  • Activated sludge
  • Microbial consortium
  • Kinetic mode
  • Dynamic mode
  • Wastewater
  • BOD sensor


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