Abstract
Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. environment, foodstuffs, clinical, pharmaceutical, diagnosis, etc.). Recently, the immuno-affinity interaction was exploited also in the sample preparation area, leading to techniques such as immuno-affinity extraction, immuno-affinity precipitation and immuno-affinity filtration, and a substantial development of an area that for a long time was neglected.
In this context, a novel immuno-affinity based sample preparation technique called Immuno Supported Liquid Membrane (ISLM) extraction is presented in this thesis. The technique allows sample cleanup and analyte enrichment in a single device that comprises both the extraction and immunoassay (imuno extraction-assay). The thesis details the development, optimisation as well as implementation aspects of the ISLM extraction, demonstrating the versatility of the technique.
In this context, a novel immuno-affinity based sample preparation technique called Immuno Supported Liquid Membrane (ISLM) extraction is presented in this thesis. The technique allows sample cleanup and analyte enrichment in a single device that comprises both the extraction and immunoassay (imuno extraction-assay). The thesis details the development, optimisation as well as implementation aspects of the ISLM extraction, demonstrating the versatility of the technique.
| Original language | English |
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| Qualification | Doctor |
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| Supervisors/Advisors |
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| Award date | 2005 Sept 2 |
| Publisher | |
| ISBN (Print) | 91-7422-089-6 |
| Publication status | Published - 2005 |
Bibliographical note
Defence detailsDate: 2005-09-02
Time: 10:30
Place: Centre for Chemistry and Chemical Engineering Sölvegatan 39 Lund
External reviewer(s)
Name: Hennion, Marie-Claire
Title: Professor Doctor
Affiliation: ESPCI (Ecole Supérieure de Physqiue et de Chimie de Paris), Laboratoire Environnement et Chimie Anal
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The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
Subject classification (UKÄ)
- Analytical Chemistry
Free keywords
- immuno supported liquid membrane (ISLM) extraction
- sample preparation
- immunoassay
- immuno-affinity interaction
- antibody
- Analytical chemistry
- Analytisk kemi