Immunocytochemical demonstration of estrogen receptor beta in human periodontal ligament cells.

Daniel Jönsson; G Andersson; Eva Ekblad; Min Liang; G Bratthall; Bengt-Olof Nilsson

doi:10.1016/S0003-9969(03)00198-5

Immunocytochemical demonstration of estrogen receptor beta in human periodontal ligament cells.

Daniel Jönsson, G Andersson, Eva Ekblad, Min Liang, G Bratthall, Bengt-Olof Nilsson

Vascular Physiology

Research output: Contribution to journal › Article › peer-review

Abstract

Two transcription associated estrogen receptor (ER) subtypes have been identified and named ERα and ERβ. In the present study we investigate the expression of these ER subtypes in cultured human periodontal ligament (PDL) cells by immunocytochemistry. ERβ immunoreactivity was observed in the nuclei of about 40% of the PDL cells, while no ERα immunoreactivity was detected. In human breast cancer MCF-7 cells, serving as positive controls, both ERα and ERβ immunoreactivities were demonstrated. No immunoreactivity was observed after omission of the primary antibodies. This study suggests that estrogen acts on gene transcription preferentially via ERβ in human PDL cells.

Original language	English
Pages (from-to)	85-88
Journal	Archives of Oral Biology
Volume	49
Issue number	1
DOIs	https://doi.org/10.1016/S0003-9969(03)00198-5
Publication status	Published - 2004

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008), Vascular Physiology (013212034), Neurogastroenterology (013212009)

Subject classification (UKÄ)

Cell and Molecular Biology
Gastroenterology and Hepatology
Physiology

Free keywords

Periodontal ligament cells
Estrogen receptors
Immunocytochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0003-9969(03)00198-5

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14693201&dopt=Abstract

Cite this

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title = "Immunocytochemical demonstration of estrogen receptor beta in human periodontal ligament cells.",

abstract = "Two transcription associated estrogen receptor (ER) subtypes have been identified and named ERα and ERβ. In the present study we investigate the expression of these ER subtypes in cultured human periodontal ligament (PDL) cells by immunocytochemistry. ERβ immunoreactivity was observed in the nuclei of about 40% of the PDL cells, while no ERα immunoreactivity was detected. In human breast cancer MCF-7 cells, serving as positive controls, both ERα and ERβ immunoreactivities were demonstrated. No immunoreactivity was observed after omission of the primary antibodies. This study suggests that estrogen acts on gene transcription preferentially via ERβ in human PDL cells.",

keywords = "Periodontal ligament cells, Estrogen receptors, Immunocytochemistry",

author = "Daniel J{\"o}nsson and G Andersson and Eva Ekblad and Min Liang and G Bratthall and Bengt-Olof Nilsson",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008), Vascular Physiology (013212034), Neurogastroenterology (013212009)",

year = "2004",

doi = "10.1016/S0003-9969(03)00198-5",

language = "English",

volume = "49",

pages = "85--88",

journal = "Archives of Oral Biology",

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T1 - Immunocytochemical demonstration of estrogen receptor beta in human periodontal ligament cells.

AU - Jönsson, Daniel

AU - Andersson, G

AU - Ekblad, Eva

AU - Liang, Min

AU - Bratthall, G

AU - Nilsson, Bengt-Olof

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008), Vascular Physiology (013212034), Neurogastroenterology (013212009)

PY - 2004

Y1 - 2004

N2 - Two transcription associated estrogen receptor (ER) subtypes have been identified and named ERα and ERβ. In the present study we investigate the expression of these ER subtypes in cultured human periodontal ligament (PDL) cells by immunocytochemistry. ERβ immunoreactivity was observed in the nuclei of about 40% of the PDL cells, while no ERα immunoreactivity was detected. In human breast cancer MCF-7 cells, serving as positive controls, both ERα and ERβ immunoreactivities were demonstrated. No immunoreactivity was observed after omission of the primary antibodies. This study suggests that estrogen acts on gene transcription preferentially via ERβ in human PDL cells.

AB - Two transcription associated estrogen receptor (ER) subtypes have been identified and named ERα and ERβ. In the present study we investigate the expression of these ER subtypes in cultured human periodontal ligament (PDL) cells by immunocytochemistry. ERβ immunoreactivity was observed in the nuclei of about 40% of the PDL cells, while no ERα immunoreactivity was detected. In human breast cancer MCF-7 cells, serving as positive controls, both ERα and ERβ immunoreactivities were demonstrated. No immunoreactivity was observed after omission of the primary antibodies. This study suggests that estrogen acts on gene transcription preferentially via ERβ in human PDL cells.

KW - Periodontal ligament cells

KW - Estrogen receptors

KW - Immunocytochemistry

U2 - 10.1016/S0003-9969(03)00198-5

DO - 10.1016/S0003-9969(03)00198-5

M3 - Article

SN - 1879-1506

VL - 49

SP - 85

EP - 88

JO - Archives of Oral Biology

JF - Archives of Oral Biology

IS - 1

ER -

Immunocytochemical demonstration of estrogen receptor beta in human periodontal ligament cells.

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

UN SDGs

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