Improved bone-forming functionality on diameter-controlled TiO2 nanotube surface

Karla S. Brammer, Seunghan Oh, Christine J. Cobb, Lars Magnus Bjursten, Henri van der Heyde, Sungho Jin

Research output: Contribution to journalArticlepeer-review

483 Citations (SciVal)


The titanium dioxide (TiO2) nanotube surface enables significantly accelerated osteoblast adhesion and exhibits strong bonding with bone. We prepared various sizes (30-100 nm diameter) of titanium dioxide (TiO2) nanotubes on titanium substrates by anodization and investigated the osteoblast cellular behavior in response to these different nanotube sizes. The unique and striking result of this study is that a change in osteoblast behavior is obtained in a relatively narrow range of nanotube dimensions, with small diameter (similar to 30 nm) nanotubes promoting the highest degree of osteoblast adhesion, while larger diameter (70-100 nm) nanotubes elicit a lower population of cells with extremely elongated cellular morphology and much higher alkaline phosphatase levels. Increased elongation of nuclei was also observed with larger diameter nanotubes. By controlling the nanotopography, large diameter nanotubes, in the similar to 100 min regime, induced extremely elongated cellular shapes, with an aspect ratio of 11:1, which resulted in substantially enhanced up-regulation of alkaline phosphatase activity, suggesting greater bone-forming ability than nanotubes with smaller diameters. Such nanotube structures, already being a strongly osseointegrating implant material, offer encouraging implications for the development and optimization of novel orthopedics-related treatments with precise control toward desired cell and bone growth behavior. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)3215-3223
JournalActa Biomaterialia
Issue number8
Publication statusPublished - 2009

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Bioimplant Research (013242910)

Subject classification (UKÄ)

  • Medical Biotechnology


  • Alkaline
  • phosphatase activity
  • Cell elongation
  • Cell adhesion
  • TiO2 nanotubes
  • Osteoblast


Dive into the research topics of 'Improved bone-forming functionality on diameter-controlled TiO2 nanotube surface'. Together they form a unique fingerprint.

Cite this