TY - JOUR
T1 - Improved chromosome-level genome assembly of the Glanville fritillary butterfly (Melitaea cinxia) integrating Pacific Biosciences long reads and a high-density linkage map
AU - Smolander, Olli Pekka
AU - Blande, Daniel
AU - Ahola, Virpi
AU - Rastas, Pasi
AU - Tanskanen, Jaakko
AU - Kammonen, Juhana I.
AU - Oostra, Vicencio
AU - Pellegrini, Lorenzo
AU - Ikonen, Suvi
AU - Dallas, Tad
AU - Dileo, Michelle F.
AU - Duplouy, Anne
AU - Duru, Ilhan Cem
AU - Halimaa, Pauliina
AU - Kahilainen, Aapo
AU - Kuwar, Suyog S.
AU - Kärenlampi, Sirpa O.
AU - Lafuente, Elvira
AU - Luo, Shiqi
AU - Makkonen, Jenny
AU - Nair, Abhilash
AU - De La Paz Celorio-Mancera, Maria
AU - Pennanen, Ville
AU - Ruokolainen, Annukka
AU - Sundell, Tarja
AU - Tervahauta, Arja I.
AU - Twort, Victoria
AU - Van Bergen, Erik
AU - Österman-Udd, Janina
AU - Paulin, Lars
AU - Frilander, Mikko J.
AU - Auvinen, Petri
AU - Saastamoinen, Marjo
PY - 2022
Y1 - 2022
N2 - Background: The Glanville fritillary (Melitaea cinxia) butterfly is a model system for metapopulation dynamics research in fragmented landscapes. Here, we provide a chromosome-level assembly of the butterfly's genome produced from Pacific Biosciences sequencing of a pool of males, combined with a linkage map from population crosses. Results: The final assembly size of 484 Mb is an increase of 94 Mb on the previously published genome. Estimation of the completeness of the genome with BUSCO indicates that the genome contains 92-94% of the BUSCO genes in complete and single copies. We predicted 14,810 genes using the MAKER pipeline and manually curated 1,232 of these gene models. Conclusions: The genome and its annotated gene models are a valuable resource for future comparative genomics, molecular biology, transcriptome, and genetics studies on this species.
AB - Background: The Glanville fritillary (Melitaea cinxia) butterfly is a model system for metapopulation dynamics research in fragmented landscapes. Here, we provide a chromosome-level assembly of the butterfly's genome produced from Pacific Biosciences sequencing of a pool of males, combined with a linkage map from population crosses. Results: The final assembly size of 484 Mb is an increase of 94 Mb on the previously published genome. Estimation of the completeness of the genome with BUSCO indicates that the genome contains 92-94% of the BUSCO genes in complete and single copies. We predicted 14,810 genes using the MAKER pipeline and manually curated 1,232 of these gene models. Conclusions: The genome and its annotated gene models are a valuable resource for future comparative genomics, molecular biology, transcriptome, and genetics studies on this species.
KW - genome
KW - Glanville fritillary
KW - Melitaea cinxia
KW - spatial ecology
U2 - 10.1093/gigascience/giab097
DO - 10.1093/gigascience/giab097
M3 - Article
C2 - 35022701
AN - SCOPUS:85123566142
SN - 2047-217X
VL - 11
JO - GigaScience
JF - GigaScience
M1 - giab097
ER -