Improved MALDI-TOF-MS analysis of partially depolymerised methyl cellulose by reducing end derivatisation

Dane Momcilovic, Gunnar Brinkmalm, Bengt Wittgren, Karl-Gustav Wahlund

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceedingpeer-review

1 Citation (SciVal)

Abstract

The depolymerized methyl cellulose was analyzed, using improved MALDI-TOF-MS by reducing the end derivatization. The methyl cellulose was depolymerized to give methylated oligo-(1->4)-β-D-anhydroglucoses (M-oligo-AG). To reduce the errors during measurement of degree of substitution (DS), M-oligo-AG was derivatized with Girard's T reagent performed on an equimolar mixture of maltooligosaccharides. The GT-derivatized M-oligo-AG (GT-M-oligo-AG) formed singly charged molecular ions with peaks at m/z values 91 higher than the corresponding peaks of M-oligo-AG. The results show that for GT-M-oligo-AG the relative signal intensities for DP=1 and 2 were increased significantly.
Original languageEnglish
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics
PublisherAmerican Society for Mass Spectrometry, Santa Fe, NM, 87505, United States
Pages421-422
Publication statusPublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Conference

ConferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period2002/06/022002/06/06

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

  • Analytical Chemistry

Keywords

  • Methyl cellulose
  • Matrix-assisted laser desorption/ionization (MALDI)
  • Time-of flight (TOF) mass spectrometry
  • Maltooligosaccharides

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