The depolymerized methyl cellulose was analyzed, using improved MALDI-TOF-MS by reducing the end derivatization. The methyl cellulose was depolymerized to give methylated oligo-(1->4)-β-D-anhydroglucoses (M-oligo-AG). To reduce the errors during measurement of degree of substitution (DS), M-oligo-AG was derivatized with Girard's T reagent performed on an equimolar mixture of maltooligosaccharides. The GT-derivatized M-oligo-AG (GT-M-oligo-AG) formed singly charged molecular ions with peaks at m/z values 91 higher than the corresponding peaks of M-oligo-AG. The results show that for GT-M-oligo-AG the relative signal intensities for DP=1 and 2 were increased significantly.
|Title of host publication||Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics|
|Publisher||American Society for Mass Spectrometry, Santa Fe, NM, 87505, United States|
|Publication status||Published - 2002|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: 2002 Jun 2 → 2002 Jun 6
|Conference||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||2002/06/02 → 2002/06/06|
Bibliographical noteThe information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
Subject classification (UKÄ)
- Analytical Chemistry
- Methyl cellulose
- Matrix-assisted laser desorption/ionization (MALDI)
- Time-of flight (TOF) mass spectrometry